Present address: Annette N. Trombert, Centro de Genomica y Bioinformatica, Instituto de Biotecnologia, Universidad Mayor, Santiago, Chile.
Reduced invasion to human epithelial cell lines of Salmonella enterica serovar Typhi carrying S. Typhimurium sopD2
Article first published online: 1 AUG 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 322, Issue 2, pages 150–156, September 2011
How to Cite
Trombert, A. N., Rodas, P. I. and Mora, G. C. (2011), Reduced invasion to human epithelial cell lines of Salmonella enterica serovar Typhi carrying S. Typhimurium sopD2. FEMS Microbiology Letters, 322: 150–156. doi: 10.1111/j.1574-6968.2011.02347.x
Editor: Ian Henderson
- Issue published online: 11 AUG 2011
- Article first published online: 1 AUG 2011
- Accepted manuscript online: 24 JUN 2011 12:27PM EST
- Received 17 March 2011; revised 14 June 2011; accepted 20 June 2011, Final version published online 1 August 2011.
Salmonella enterica serovar Typhi and Typhimurium are closely related serovars. However, S. Typhi, a human-specific pathogen, has 5% of genes as pseudogenes, far more than S. Typhimurium, which only has 1%. One of these pseudogenes corresponds to sopD2, which in S. Typhimurium encodes an effector protein involved in Salmonella-containing vacuole biogenesis in human epithelial cell lines, which is needed for full virulence of the pathogen. We investigated whether S. Typhi trans-complemented with the functional sopD2 gene from S. Typhimurium (sopD2STM) would reduce the invasion of human epithelial cell lines. Our results showed that the presence of sopD2STM in S. Typhi significantly modified the bacterial ability to alter cellular permeability and decrease the CFUs recovered after cell invasion of human epithelial cell line. These results add to mounting evidence that pseudogenes contribute to S. Typhi adaptation to humans.