Editor: Jörg Simon
Genetic analysis of the Alteromonas macleodii [NiFe]-hydrogenase
Article first published online: 19 JUL 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 322, Issue 2, pages 180–187, September 2011
How to Cite
Weyman, P. D., Smith, H. O. and Xu, Q. (2011), Genetic analysis of the Alteromonas macleodii [NiFe]-hydrogenase. FEMS Microbiology Letters, 322: 180–187. doi: 10.1111/j.1574-6968.2011.02348.x
- Issue published online: 11 AUG 2011
- Article first published online: 19 JUL 2011
- Accepted manuscript online: 30 JUN 2011 03:59PM EST
- Received 3 May 2011; revised 20 June 2011; accepted 23 June 2011, Final version published online 19 July 2011.
- [NiFe] hydrogenase;
- physiology of H2 metabolism;
- Urania basin
Alteromonas macleodii Deep ecotype is a marine, heterotrophic, gammaproteobacterium isolated in the Mediterranean Sea between depths of 1000 and 3500 m. The sequenced strain was previously reported to contain a [NiFe] hydrogenase. We verified the presence of this hydrogenase in other strains of A. macleodii Deep ecotype that were previously isolated from several bathypelagic microenvironments. We developed a system for the genetic manipulation of A. macleodii Deep ecotype using conjugation and used this system to create mutant strains that lack the [NiFe] hydrogenase structural genes (hynSL). The mutants did not possess hydrogenase activity, and complementation of the mutant strain with the hynSL genes successfully restored hydrogenase activity. Both the mutant and the wild-type strains grew at the same rate in a variety of media and under different environmental conditions, indicating little effect of the hydrogenase mutation under the conditions tested.