Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh

Authors

  • Ying Hou,

    1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
    2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, China
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  • Jian Tao,

    1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
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  • Wenjing Shen,

    1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
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  • Juan Liu,

    1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
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  • Jingquan Li,

    1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
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  • Yongfeng Li,

    1. Institute of Plant Protection, Jiangsu Agricultural Academy, Nanjing, China
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  • Hui Cao,

    1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
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  • Zhongli Cui

    Corresponding author
    • Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China
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Correspondence: Zhongli Cui, Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China. Tel./fax: +86 25 84396753; e-mail: czl@njau.edu.cn

Abstract

An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L−1FE within 24 h and the metabolite fenoxaprop acid (FA) was identified by HPLC/MS analysis. This strain converted FE by cleavage of the ester bond, but could not further degrade FA. Strain T1 could also efficiently degrade haloxyfop-R-methyl, quizalofop-p-ethyl, cyhalofop-butyl and clodinafop-propargyl. FE hydrolase capable of hydrolysing FE to FA was found in the cell-free extract of strain T1 by zymogram analysis. A novel gene feh encoding FE hydrolase was cloned by shotgun library construction and successfully expressed in Escherichia coli.

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