Probiotic genomic DNA reduces the production of pro-inflammatory cytokine tumor necrosis factor-alpha

Authors


Correspondence: Dae Kyun Chung, School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, South Korea. Tel.: +82 31 2012465; fax: +82 31 2028333; e-mail: dkchung@khu.ac.kr

Abstract

The effect of Lactobacillus plantarum genomic DNA on lipopolysaccharide (LPS)-induced mitogen-activated protein kinase (MAPK) activation, nuclear factor-kappa B activation, and the expressions of tumor necrosis factor-alpha, interleukin-1 receptor-associated kinase M, and the pattern recognition receptor were examined. Pretreatment of p-gDNA inhibited the phosphorylation of MAPKs and nuclear factor-kappa B, and also inhibited LPS-induced TNF-α production in response to subsequent LPS stimulation. L. plantarum genomic DNA-mediated inhibition of signaling pathway and tumor necrosis factor-alpha was accompanied by the suppression of toll-like receptor (TLR) 2, TLR4, and TLR9 and the induction of interleukin-1 receptor-associated kinase M, a negative regulator of TLR. This study can extend our understanding of the biological function of probiotic genomic DNA as an anti-inflammatory agent.

Ancillary