As an opportunistic pathogen, it is not surprising that P. gingivalis possesses a number of virulence factors. These are molecules that can elicit deleterious effects on host cells, essentially the survival ‘weapons’ of P. gingivalis. The main virulence factors discussed here are LPS, capsular polysaccharide (CPS), fimbriae and gingipains.
The LPS of P. gingivalis
Like all Gram-negative bacterial species, P. gingivalis is sheathed by an LPS, which is an outer membrane component recognized by the host that can trigger intracellular signalling events. The affinity of LPS to its pattern recognition receptors, such as the TLRs and CD14, enables discrimination between commensal and pathogenic species. The P. gingivalis LPS is a stimulator of proinflammatory responses and bone resorption, as demonstrated in experimental animal models (Chiang et al., 1999; Nishida et al., 2001). In vitro, it stimulates proinflammatory cytokine production of, for example, IL-1α, IL-1β, IL-6, IL-8, IL-18 and tumour necrosis factor (TNF)-α in monocytes (Zhou et al., 2005; Bostanci et al., 2007a, b; Hamedi et al., 2009). Yet, P. gingvalis LPS exhibits controversial features with regard to the induction of an inflammatory response. Apart from being a weaker cytokine stimulator compared with the LPS of other Gram-negative (i.e. enteropathogenic) species (Liu et al., 2008), it can also antagonize the cytokine-stimulating capacity of other putative pathogens (Bostanci et al., 2007a, b).
Structurally, P. gingivalis LPS exhibits unique features compared with the LPS of other species. These include differences in the structure of the O-antigen between P. gingivalis strains that can confer antigenic differences (Paramonov et al., 2001, 2009), as well as in the acylation patterns and receptor-activating capacities of the lipid A component. While the lipid A of most Gram-negative species is a strong activator of TLR4 responses, P. gingivalis lipid A is predominantly a TLR2 activator and may even act as antagonist to TLR4 (Darveau et al., 2004), dampening the immune responses (Hajishengallis, 2009). When considering further the heterogeneous acylation patterns of P. gingivalis lipid A, two forms are predominant: the tetra-acylated and penta-acylated forms. These two structures induce opposing host responses. The penta-acylated lipid A activates TLR4, whereas tetra-acylated lipid A acts as a TLR4 antagonist (Darveau et al., 2004; Nemoto et al., 2006). These changes of P. gingivalis lipid A acylation are dependent on microenvironmental conditions. In particular, when hemin availability is high (a condition that reflects inflammation), penta-acylated lipid A is converted into tetra-acylated lipid A (Al-Qutub et al., 2006). Hence, by modifying its lipid A structure according to the microenvironment, P. gingivalis may modulate the binding affinity of its LPS to its cognate TLR receptors, subsequently selecting how to affect downstream host immune signalling. Interestingly, a second type of LPS has also been identified in P. gingivalis, containing a distinct anionic polysaccharide linked to lipid A, known as A-LPS (Paramonov et al., 2005). A-LPS is required for cell integrity and serum resistance (Shoji et al., 2002; Paramonov et al., 2005; Slaney et al., 2006) and is structurally associated with the Arg-X gingipain (Curtis et al., 1999; Paramonov et al., 2005). It is also a weaker inducer of cytokine responses by human monocytes, as compared with the conventional LPS (Rangarajan et al., 2008). Collectively, the modifications and heterogeneity of P. gingivalis LPS can result in opposing actions and immunological deregulation. Strategically, this is in line with the manipulation of host innate immune responses by this species, to facilitate its adaptation and survival into the host.
The CPS of P. gingivalis
A major virulence factor of P. gingivalis is considered to be its capsule, also known as CPS or K-antigen (Schifferle et al., 1989; Holt et al., 1999; Farquharson et al., 2000; Aduse-Opoku et al., 2006; Brunner et al., 2010a, b). Based on the capacity of CPS to generate systemic IgG antibody responses, at least six different serotypes have been identified (Laine et al., 1997; Sims et al., 2001). Encapsulated P. gingivalis strains are shown to be highly invasive, causing spreading infection in a murine lesion model, whereas nonencapsulated strains induced only localized abscesses (Laine & van Winkelhoff, 1998). Interestingly, immunization with P. gingivalis CPS induced a high IgG systemic response (Choi et al., 1998) and reduced P. gingivalis-induced alveolar bone loss (Gonzalez et al., 2003). Encapsulated strains of P. gingivalis are more resistant to phagocytosis by polymorphonuclear leukocytes than nonencapsulated strains (Sundqvist et al., 1991) and have differential capacities to adhere to gingival epithelial cells (Dierickx et al., 2003). Moreover, differences in CPS serotypes can reflect differential capacities in chemokine stimulation by macrophages (d'Empaire et al., 2006) or cytokine stimulation by dendritic cells (Vernal et al., 2009). Interestingly, a nonencapsulated P. gingivalis knockout mutant strain was found to be a more potent inducer of cytokine synthesis by human gingival fibroblasts, as compared with the corresponding wild-type strain, implying a role of CPS in downplaying the innate immune responses (Brunner et al., 2010a, b). Although it is evident that the presence of CPS, or its individual serotypes, could be determinants of the virulence of P. gingivalis, the potential involvement of this antigen in the overall deregulation of host responses awaits further clarification.
The fimbriae of P. gingivalis
The fimbriae of P. gingivalis are thin, filamentous cell-surface protrusions that facilitate its adherence to salivary proteins, extracellular matrix, eukaryotic cells and bacteria of either the same or other species. Through its fimbriae, P. gingivalis can thus attach to early colonizing bacteria, and participate in the developing biofilm structure. Type I (major) fimbriae have important roles in colonization and invasion, whereas type II (minor) fimbriae possess a higher proinflammatory capacity (Lamont & Jenkinson, 1998; Amano et al., 2004; Hajishengallis et al., 2008). Interestingly, however, P. gingivalis strains W50 and W83 that lack major fimbriae are still invasive, as demonstrated in experimental subcutaneous abscess models (Inaba et al., 2008). A particular role of fimbriae is revealed in the induction of bone destruction in experimental periodontitis models. To this extent, infection of rats with nonfimbriated P. gingivalis strains exhibited reduced periodontal bone loss, compared with infection with fimbriated strains (Jotwani & Cutler, 2004). Moreover, immunization against P. gingivalis fimbriae protected against bone loss in gnothobiotic rats (Malek et al., 1994; Sharma et al., 2001). Other properties of both major and minor fimbriae are the induction of proinflammatory cytokines and production of matrix metalloproteinases (MMPs), such as IL-1, IL-6, IL-8, TNF-α and MMP-9, by various host cells (Jotwani et al., 2010; Ogawa et al., 1994; Pollreisz et al., 2010; Takahashi et al., 2006).
Porphyromonas gingivalis fimbriae can signal through either TLR2 or TLR4. Activation of TLR2 by fimbriae results in a differential signalling pattern compared with activation by P. gingivalis LPS (Hajishengallis et al., 2006). Fimbriae can directly induce two distinct signalling pathways, one that mediates production of proinflammatory cytokines, such as IL-6 and TNF-α, and another that mediates the expression of cell adhesion molecules, such as ICAM-1 (Hajishengallis et al., 2009). On the other hand, signalling through TLR4 requires an additional costimulation of CD14 and MD-2 (Davey et al., 2008). Interestingly, major fimbriae can exploit TLR2 signalling in order to interact with complement receptor 3 (CR3), in a novel ‘inside-out’ signalling pattern (Hajishengallis et al., 2007; Wang et al., 2007). This interaction activates the binding capacity of CR3 and allows for internalization of P. gingivalis in macrophages and reduction of IL-12 production, which may collectively inhibit bacterial clearance (Hajishengallis et al., 2007).
The gingipains of P. gingivalis
Gingipains are a group of cell surface cysteine proteinases of P. gingivalis that can also be present in secreted soluble form. They account for 85% of the total proteolytic activity of P. gingivalis (Potempa et al., 1997). Based on their substrate specificity, they are divided into arginine-specific (Arg-X) and lysine-specific (Lys-X) gingipains (Curtis et al., 2001; Guo et al., 2010). Arg-X gingipains have trypsin-like activity, and can degrade extracellular matrix components, including the integrin–fibronectin-binding, cytokine, immunoglobulin and complement factors. There are two types of Arg-X gingipains, namely RgpA, which contains a proteolytic and an adhesion domain, and RgpB, which contains only the proteolytic domain. There is one type of Lys-X gingipain, Kgp, which contains both a proteolytic and an adhesion domain. There are sequence similarities between the adhesion domains of Kgp and RgpA (Curtis et al., 2001).
The gingipains have multiple effects on the molecular components of the immune response, and as such they can deregulate these responses. For instance, they can cleave several T-cell receptors, such as CD2, CD4 and CD8 (Kitamura et al., 2002), thereby hampering the cell-mediated immune response. They can also stimulate expression of protease-activated receptors in neutrophils (Lourbakos et al., 1998), gingival epithelial cells (Lourbakos et al., 2001), gingival fibroblasts and T cells (Belibasakis et al., 2010), which are crucial for the induction of cytokine responses and the establishment of chronic inflammation in periodontitis (Holzhausen et al., 2010; Fagundes et al., 2011). Gingipains can also stimulate IL-6 production by oral epithelial cells (Lourbakos et al., 2001) and IL-8 production by gingival fibroblasts (Oido-Mori et al., 2001), enhancing the inflammatory responses. However, they can also proteolytically inactivate both anti-inflammatory (IL-4, IL-5) and pro-inflammatory (IL-12, IFN-γ) cytokines (Yun et al., 1999, 2001, 2002; Tam et al., 2009).
A number of particularly interesting effects are exerted by the gingipains on components of the complement system. Arg-X gingipains can cleave the C5 molecule, resulting in release of its C5a component, which is crucial for enhancing the recruitment of PMNs (Wingrove et al., 1992; Imamura et al., 2001). On the other hand, Lys-X can inactivate the C5a receptor on PMNs, an action that may actually impair their recruitment (Jagels et al., 1996a, b). Along this line, the Arg-X gingipains can degrade the C3 molecule, potentially contributing to decreased bacterial opsonization (Schenkein et al., 1995). This property could confer increased resistance of P. gingivalis to bactericidal activity.
Apart from their effect on immune responses, gingipains may also be involved in the binding of P. gingivalis to host cells, as Rgp–Kgp complexes have been shown to mediate adherence on gingival epithelial cells and gingival fibroblasts (Chen et al., 2001; Grenier et al., 2003; Andrian et al., 2004). Interestingly, when P. gingivalis intracellularly invades gingival epithelial cells, expression of gingipain is downregulated (Xia et al., 2007).
Gingipains may also affect vascular permeability and bleeding at the periodontal site. They can proteolytically activate plasma kallikrein and bradykinin, or alternatively increase the release of thrombin and prothrombin, which can result in increased vascular permeability and PMN influx (Imamura et al., 1994, 1995a). Moreover, by degrading fibrinogen (Scott et al., 1993), they may contribute to inhibition of blood coagulation and increase bleeding at the site (Imamura et al., 1995a) , thus enhancing the availability of hemin required for P. gingivalis growth.
Collectively, studies in various experimental systems indicate that gingipains have seemingly contradicting actions on the innate immune responses, hampering interpretation of their role in the pathogenesis of periodontitis. Nevertheless, such differences may be reconciled by the existence of a concentration gradient of gingipains in the tissue (Pathirana et al., 2010). Closer to the gingival epithelial barrier where the biofilm resides, gingipain concentrations are high, causing degradation or deregulation of various components of the immune response. This hampers bacterial clearance and facilitates bacterial invasion. Nevertheless, deeper into the gingival connective tissue, gingipain concentrations become gradually lower and stimulate, rather than inhibit, inflammation. This may in turn induce connective tissue and bone destruction, which are the hallmarks of periodontitis.