Research Letter

Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus

  1. Els Van Pamel1,*,
  2. Els Daeseleire1,
  3. Nikki De Clercq1,
  4. Lieve Herman1,
  5. Annemieke Verbeken2,
  6. Marc Heyndrickx1,
  7. Geertrui Vlaemynck1

Article first published online: 18 JUN 2012

DOI: 10.1111/j.1574-6968.2012.02608.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 333, Issue 2, pages 153–159, August 2012

Additional Information

How to Cite

Van Pamel, E., Daeseleire, E., De Clercq, N., Herman, L., Verbeken, A., Heyndrickx, M. and Vlaemynck, G. (2012), Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus. FEMS Microbiology Letters, 333: 153–159. doi: 10.1111/j.1574-6968.2012.02608.x

Author Information

  1. 1

    Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Melle, Belgium

  2. 2

    Mycology Research Group, Department of Biology, Ghent University, Ghent, Belgium

*Correspondence: Els Van Pamel, Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, 9090 Melle, Belgium. Tel.: +32 9 272 30 12; fax: +32 9 272 30 01; e-mail: els.vanpamel@ilvo.vlaanderen.be

Publication History

  1. Issue published online: 23 JUL 2012
  2. Article first published online: 18 JUN 2012
  3. Accepted manuscript online: 2 JUN 2012 06:02AM EST
  4. Manuscript Accepted: 24 MAY 2012
  5. Manuscript Revised: 6 FEB 2012
  6. Manuscript Received: 9 JAN 2011

Funded by

  • Institute for the Promotion of Innovation through Science and Technology. Grant Number: IWT-SB/63435

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Keywords:

  • Aspergillus fumigatus;
  • rodA gene;
  • restriction analysis

Abstract

A previous multidisciplinary study indicated that gliotoxin-producing Aspergillus fumigatus Fresen. isolates from silage commodities mostly belonged to its variant A. fumigatus var. ellipticus Raper & Fennell. Sequence analysis revealed the presence of a single nucleotide polymorphism at five positions in a fragment of the rodA gene (coding for a hydrophobin rodletA protein) between Aspergillus fumigatus var. fumigatus and Aspergillus fumigatus var. ellipticus. A method was developed to distinguish these two types of isolates based on restriction analysis of this rodA gene fragment using the HinfI restriction enzyme. In addition, in silico analysis of 113 rodA gene fragments retrieved from GenBank was performed and confirmed the suitability of this method. In conclusion, the method developed in this study allows easy distinction between A. fumigatus var. fumigatus and its variant ellipticus. In combination with the earlier developed PCR-restriction fragment length polymorphism method of Staab et al. (2009, J Clin Microbiol 47: 2079), this method is part of a sequencing-independent identification scheme that allows for rapid distinction between similar species/variants within Aspergillus section Fumigati, specifically A. fumigatus,A. fumigatus var. ellipticus,Aspergillus lentulus Balajee & K.A. Marr, Neosartorya pseudofischeri S.W. Peterson and Neosartorya udagawae Y. Horie, Miyaji & Nishim.

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