• 1
    Brown, A.D. (1976) Microbiol water stress. Bacteriol. Rev. 40, 803846.
  • 2
    Brown, A.D. (1978) Compatible solutes and extreme water stress in eukaryotic micro-organisms. Adv. Microb. Physiol. 17, 181242.
  • 3
    Reed, R.H., Chudek, J.A., Foster, R. and Gadd, G.M. (1987) Osmotic significance of glycerol accumulation in exponentially growing yeasts. Appl. Environ. Microbiol. 53, 21192123.
  • 4
    Bellinger, Y. and Larher, F. (1987) A 13C comparative nuclear magnetic resonance study of organic solute production and excretion by the yeasts Hansenula anomala and Saccharomyces cerevisiae in saline media. Can. J. Microbiol. 34, 605612.
  • 5
    Meikle, A.J., Reed, R.H. and Gadd, G.M. (1991) The osmotic response of Saccharomyces cerevisiae in K+-depleted medium. FEMS Microbiol. Lett. 78, 8994.
  • 6
    Blomberg, A. and Adler, L. (1992) Physiology of osmotolerance in fungi. Adv. Microb. Physiol. 33, 145212.
  • 7
    Prior, B.A. and Hohmann, S. (1997) Glycerol production and osmoregulation. In: Yeast Sugar Metabolism (Zimmermann, F.K. and Entian, K.-D., Eds.), pp. 313–337. Technomic, Lancaster.
  • 8
    Hohmann, S. (1997) Shaping up: The response of yeast to osmotic stress. In: Yeast Stress Responses (Hohmann, S. and Mager, W.H., Eds.), pp. 101–145. Springer-Verlag, Heidelberg.
  • 9
    Barnett, J.A., Payne, R.W. and Yarrow, D.Y. (1983). Yeast: Characteristics and Identification. Cambridge University Press, Cambridge.
  • 10
    Van Dijken, J.P. and Scheffers, W.A. (1986) Redox balances in the metabolism of sugars by yeasts. FEMS Microbiol. Rev. 32, 199224.
  • 11
    Ansell, R., Granath, K., Hohmann, S., Thevelein, J.M. and Adler, L. (1997) The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J. 16, 21792187.
  • 12
    Thevelein, J.M. and Hohmann, S. (1995) Trehalose synthase, guard to the gate of glycolysis in yeast? Trends Biochem. Sci. 20, 310.
  • 13
    Paltauf, F., Kohlwein, S.D. and Henry, S.A. (1992) Regulation and compartmentalization of lipid synthesis in yeast. In: The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae (Jones, E.W., Pringle, J.R. and Broach, J.R., Eds.), Vol. 2, pp. 415–500. Cold Spring Harbor Laboratory Press, Cold spring Harbor, NY.
  • 14
    Gancedo, C., Gancedo, J.M. and Sols, A. (1968) Glycerol metabolism in yeasts. Eur. J. Biochem. 5, 165172.
  • 15
    Klingenberg, M. (1970) Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur. J. Biochem. 13, 247252.
  • 16
    Sprague, G.F. and Cronan, J.E. (1977) Isolation and characterization of Saccharomyces cerevisiae mutants defective in glycerol catabolism. J. Bacteriol. 129, 13351342.
  • 17
    Pavlik, P., Simon, M. and Ruis, H. (1993) The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization. Curr. Genet. 24, 2125.
  • 18
    Rönnow, B. and Kielland-Brandt, M.C. (1993) GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae. Yeast 9, 11211130.
  • 19
    Babel, W. and Hofmann, K.H. (1982) The relation between the assimilation of methanol and glycerol in yeasts. Arch. Microbiol. 132, 179184.
  • 20
    May, J.W., Marshall, J.H. and Sloan, J. (1982) Glycerol utilization by Schizosaccharomyces pombe: phosphorylation of dihydroxyacetone by a specific kinase as the second step. J. Gen. Microbiol. 128, 17631766.
  • 21
    Norbeck, J. and Blomberg, A. (1997) Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. J. Biol. Chem. 272, 55445554.
  • 22
    Larsson, K., Ansell, R., Eriksson and P. Adler, L. (1993) A gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae. Mol. Microbiol. 10, 11011111.
  • 23
    Albertyn, J., Hohmann, S., Thevelein, J.M. and Prior, B.A. (1994) GPD1 encoding glycerol 3-phosphate dehydrogenase is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol. Cell. Biol. 14, 41354144.
  • 24
    Wang, H.-T., Rahaim, P., Robbins, P. and Yocum, R.R. (1994) Cloning, sequence and disruption of the Saccharomyces diastaticus DAR1 gene encoding a glycerol 3-phosphate dehydrogenase. J. Bacteriol. 176, 70917095.
  • 25
    Eriksson, P., Andre, L., Ansell, R., Blomberg, A. and Adler, L. (1995) Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) in, and its comparison with GPD1. Mol. Microbiol. 17, 95107.
  • 26
    Norbeck, J., Pahlman, A.-K., Akhtar, N., Blomberg, A. and Adler, L. (1996) Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. J. Biol. Chem. 271, 1387513881.
  • 27
    Andre, L., Hemming, A. and Adler, L. (1991) Osmoregulation in Saccharomyces cerevisiae: Studies on the osmotic induction of glycerol production and glycerol 3-phosphate dehydrogenase (NAD+). FEBS Lett. 286, 1317.
  • 28
    Blomberg, A., Larsson, C. and Gustafsson, L. (1988) Microcalorimetric monitoring of growth of Saccharomyces cerevisiae: osmotolerance in relation to physiological state. J. Bacteriol. 170, 45624568.
  • 29
    Albertyn, J., Hohmann, S. and Prior, B.A. (1994) Characterization of the osmotic-stress response in S. cerevisiae: osmotic stress and glucose repression regulate glycerol 3-phosphate dehydrogenase independently. Curr. Genet. 25, 1218.
  • 30
    Polakis, E.S., Bartley, W. and Meek, G.A. (1965) Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources. Biochem. J. 97, 298302.
  • 31
    De Vries, S. and Marres, V. (1987) The mitochondrial respiratory chain of yeast. Structure and biosynthesis and the role in cellular metabolism. Biochim. Biophys. Acta 895, 205239.
  • 32
    Sleep, D., Ogden, J.E., Roberts, N.A. and Goodey, A.R. (1991) Cloning and characterisation of the Saccharomyces cerevisiae glycerol 3-phosphate dehydrogenase (GUT2) promoter. Gene 101, 8996.
  • 33
    Blomberg, A. and Adler, L. (1989) Roles of glycerol and glycerol 3-phosphate dehydrogenase (NAD+) in acquired osmotolerance of Saccharomyces cerevisiae. J. Bacteriol. 171, 10871092.
  • 34
    Garcia, M.J., Rios, G., Ali, R., Belles, J.M. and Serrano, R. (1997) Comparative physiology of salt tolerance in Candida tropicalis and Saccharomyces cerevisiae. Microbiology 143, 11251131.
  • 35
    Meikle, A.J., Chudek, J.A., Reed, R.H. and Gadd, G.M. (1988) Natural abundance 13C-nuclear magnetic resonance spectroscopic analysis of acrylic polyol and trehalose accumulation by several yeast species in response to salt stress. FEMS Microbiol. Lett. 82, 163168.
  • 36
    Gancedo, C. and Serrano, R. (1989) Energy-yielding metabolism. In: The Yeasts (Rose, A.H. and Harrison, J.S., Eds.), Vol. 3, pp. 205–259. Academic Press, London.
  • 37
    Latterich, M. and Watson, M.D. (1993) Evidence for a dual osmoregulatory mechanism in the yeast Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 191, 11111117.
  • 38
    Edgley, M. and Brown, A.D. (1983) Yeast water relations: physiological changes induced by solute stress in Saccharomyces cerevisiae and Saccharomyces rouxii. J. Gen. Microbiol. 129, 34533463.
  • 39
    Singh, K.K. and Norton, R.S. (1991) Metabolic changes induced during adaption of Saccharomyces cerevisiae to a water stress. Arch. Microbiol. 156, 3842.
  • 40
    Mackenzie, K.F., Blomberg, A. and Brown, A.D. (1986) Water stress plating hypersensitivity of yeasts. J. Gen. Microbiol. 132, 20532056.
  • 41
    Blomberg, A. (1997) The osmotic hypersensitivity of the yeast Saccharomyces cerevisiae is strain and growth media dependent: Quantitative aspects of the phenomenon. Yeast 13, 529539.
  • 42
    Varela, J.C.S., van Beekvelt, C., Planta, R.J. and Mager, W.H. (1992) Osmostress-induced changes in yeast gene expression. Mol. Microbiol. 6, 21832190.
  • 43
    Hirayama, T., Maeda, T., Saito, H. and Shinozaki, K. (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol. Gen. Genet. 249, 127138.
  • 44
    Blomberg, A. (1995) Global changes in protein synthesis during adaption of the yeast Saccharomyces cerevisiae to 0.7 M NaCl. J. Bacteriol. 177, 35633572.
  • 45
    Nevoigt, E. and Stahl, U. (1996) Reduced pyruvate decarboxylase (PDC) and increased glycerol 3-phosphate dehydrogenase [NAD+] (GPD) levels enhance glycerol production in Saccharomyces cerevisiae. Yeast 12, 13311337.
  • 46
    Norbeck, J. and Blomberg, A. (1996) Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae. FEMS Microbiol. Lett. 137, 18.
  • 47
    Miralles, V.J. and Serrano, R. (1995) A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress. Mol. Microbiol. 17, 653662.
  • 48
    Van Zyl, P.J., Prior, B.A. and Kilian, S.G. (1991) Regulation of glycerol metabolism in Zygosaccharomyces rouxii in response to osmotic stress. Appl. Microbiol. Biotechnol. 36, 369374.
  • 49
    Adler, L., Blomberg, A. and Nilsson, A. (1985) Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii. J. Bacteriol. 162, 300306.
  • 50
    De Gier, J., Mandersloot, J.G. and Van Deenen, L.L.M. (1968) Lipid composition and permeability of liposomes. Biochim. Biophys. Acta 150, 666675.
  • 51
    Thunblad-Johansson, I. and Adler, L. (1987) Effects of sodium chloride concentration on phospholipid fatty acid composition of yeast differing in osmotolerance. FEMS Microbiol. Lett. 43, 275278.
  • 52
    Luyten, K., Albertyn, J., Skibbe, W.F., Prior, B.A., Ramos, J., Thevelein, J.M. and Hohmann, S. (1995) Fps1, a yeast member of the MIP family of channel proteins, is a facilitator for glycerol uptake and efflux and is inactive under osmotic stress. EMBO J. 14, 13601371.
  • 53
    Van Aelst, L., Hohmann, S., Zimmermann, F.K., Jans, A.W.H. and Thevelein, J.M. (1991) A yeast homologue of bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling. EMBO J. 10, 20952104.
  • 54
    Van Zyl, P.J., Kilian S.G. and Prior, P.A. (1990) The role of an active transport mechanism in glycerol accumulation during osmoregulation by Zygosaccharomyces rouxii. Appl. Microbiol. Biotechnol. 34, 231235.
  • 55
    Maeda, T., Wurgler-Murphy, S.M. and Saito, H. (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369, 242245.
  • 56
    Maeda, T., Takekawa, M. and Saito, H. (1995) Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor. Science 269, 554558.
  • 57
    Posas, F., Wurgler-Murphy, S.M., Maeda, T., Witten, E.A., Thai, T.C. and Saito, H. (1996) Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 ‘two-component’ osmosensor. Cell 86, 865875.
  • 58
    Posas, F. and Saito, H. (1997) Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: Scaffold role of Pbs2p MAPKK. Science 276, 17021705.
  • 59
    Brewster, J.L., de Valoir, T., Dwyer, N.D., Winter, E. and Gustin, M.C. (1993) An osmosensing signal transduction pathway in yeast. Science 259, 17601763.
  • 60
    Schüller, C., Brewster, J.L., Alexander, M.R., Gustin, M.C. and Ruis, H. (1994) The HOG pathway controls osmotic regulation of transcription via the stress responsive element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J. 13, 43824389.
  • 61
    Mager, W.H. and De Kruijff, A.J.J. (1995) Stress-induced transcriptional activation. Microbiol. Rev. 59, 506531.
  • 62
    Akhtar, N., Blomberg, A. and Adler, L. (1997) Osmoregulation and protein expression in a pbs2Δ mutant of Saccharomyces cerevisiae during adaptation to hypersaline stress. FEBS Lett. 403, 173180.
  • 63
    Gustin, M.C., Zhou, X.-L., Martinac, B. and Kung, C. (1988) A mechanosensitive ion channel in the yeast plasma membrane. Science 242, 762765.
  • 64
    Herskowitz, I. (1995) MAP kinase pathways in yeast: For mating and more. Cell 80, 187197.
  • 65
    Treisman, R. (1996) Regulation of transcription by MAP kinase cascades. Curr. Opin. Cell. Biol. 8, 205215.
  • 66
    Varela, J.C.S. and Mager, W.H. (1996) Response of Saccharomyces cerevisiae to changes in external osmolarity. Microbiology 142, 721731.
  • 67
    Davenport, K.R., Sohaskey, M., Kamada, Y., Levin, D.E. and Gustin, M.C. (1995) A second osmosensing signal transduction pathway in yeast. J. Biol. Chem. 270, 3015730161.