Dedicated to Prof. Dr. Otto Kandler on the occasion of his 80th birthday.
New techniques for isolation of single prokaryotic cells1
Article first published online: 9 JAN 2006
FEMS Microbiology Reviews
Volume 24, Issue 5, pages 567–572, December 2000
How to Cite
Fröhlich, J. and König, H. (2000), New techniques for isolation of single prokaryotic cells. FEMS Microbiology Reviews, 24: 567–572. doi: 10.1111/j.1574-6976.2000.tb00558.x
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 7 March 2000, Revised 12 July 2000, Accepted 28 July 2000
- Optical tweezer;
- Laser microdissection;
- Single microbial cell
Since the 1960s, several new attempts have been made to improve the management of single prokaryotic cells using micromanipulator techniques. In order to facilitate the isolation of pure cultures we have recently developed an improved micromanipulation method for routine work. With the aid of this method single prokaryotic cells can be picked out of a mixed community under direct visual control. The isolated aerobic or anaerobic cells can be grown in pure culture or can be subjected to single cell PCR. Other powerful and completely new approaches are the applications of laser micromanipulation systems, such as optical tweezers or laser microdissection techniques. Of the latter two methods only optical tweezers have been successfully applied to cloning prokaryotic cells.