Origin^{a} | Biochemical properties | Specific substrates^{b} | Remarks | Reference |

*B. gladioli* ATCC10248 (EstB) | 392 aa, 42 kDa | pNP-esters, triglycerides (up to C_{6}), deacylates, cephalosporins | S-x-x-K motif, β-lactamase-like | [9] |

*B. gladioli* ATCC10248 (EstC) | 298 aa, 32 kDa | pNP-esters (up to C_{5}), not triglycerides | G-x-S-x-G motif, homology to plant hydroxynitrile lyases | [65] |

*P. fluorescens* DSM50106 | 36 kDa, T_{opt.} 43°C | Lactones, ethyl caprylate, moderate enantioselectivity | G-x-S-x-G motif, homology to a haloperoxidase | [37] |

*P. fluorescens* SIKW1 | 27 kDa, homodimer | pNP-esters, high enantioselectivity for α-phenyl ethanol, moderate *E* values for other alcohols and carboxylic acids | Low haloperoxidase activity, altered substrate specificity and improved enantioselectivity by directed evolution | [12,49,50,53,60,61,66] |

*Pseudomonas putida* MR2068 | 29 kDa, homodimer, T_{opt.} 70°C | Alkyl-dicarboxylic acid methylesters, high stereoselectivity (*E*>100) | – | [58,67] |

*Bacillus acidocaldarius* | 34 kDa, T_{opt.} 70°C | pNP-esters (best: hexanoate), moderate stereoselectivity (best: *E*∼18) | Homology to hormone-sensitive lipase | [68] |

*B. subtilis* NRRL B8079 | 489 aa, 54 kDa, T_{opt.} 52°C (66.5°C for best mutant) | *p*-nitrobenzyl ester of Loracarbef | Evolved by directed evolution for increased stability in DMF and thermostability | [16,17,38,55] |

*Bacillus stearothermophilus* | – | pNP-esters, moderate enantioselectivity | Thermostable mutants | [69,70] |

*B. subtilis* (ThaiI-8) ^{c} | 32 kDa, T_{opt.} 35–55°C | High enantioselectivity towards 2-arylpropionic acids | Structure known, more stable mutants by SDM^{b} | [20] |

*Thermoanaerobacterium* sp. JW/SL YS485 | 320 aa, 36 kDa | Xylose tetra acetate, cephalosporin C, MU-Ace^{b} | G-x-S-x-G motif | [35] |

*Acinetobacter* sp. ADP1 | 37 kDa | pNP-esters (best: hexanoate), benzyl esters | G-x-S-x-G motif, involved in catechol branch of β-ketoadipate pathway | [36] |

*Clostridium thermocellum* | 31 kDa | Feruloyl esters | Esterase activity within cellulosome | [33] |

*Pyrococcus furiosus* DSM3638 | T_{opt.} 100°C, *t*_{1/2} 50 min at 126°C | MU-Ace^{b} | – | [71] |

*Lactococcus lactis*^{e} | 258 aa, 30 kDa | pNP-esters (best: hexanoate), tributyrin, C_{6}-phospholipids | G-x-S-x-G motif, function unclear | [72] |

*Rhodococcus ruber* DSM 43338^{d} | Two esterases with opposite enantiopreference | Linalool-acetate (*E*>100) | Two esterases with opposite enantiopreference | [59] |

*Rhodococcus* sp. H1 | 34 kDa, tetramer | Heroin | G-x-S-x-G motif, conserved His_{86} | [73] |

*Rhodococcus* sp. MB1 | 574 aa, 65 kDa, monomer | Cocaine | G-x-S-Y-x-G motif, homology to X-prolyl-dipeptidyl aminopeptidases | [74] |

*Streptomyces chrysomallus* | 42 kDa | pNP-esters (best: butyrate) | G-x-S-x-G motif, high homology to esterase from *A. globiformis* | [75] |

*Streptomyces diastatochromogenes* | 326 aa, 31 kDa | pNP-esters, moderate enantioselectivity | – | [76,77] |

*Orpinomyces* sp. PC-2 | 313 aa, 35 kDa, T_{opt.} 30°C | Xylose tetra acetate | High homology to other acetyl xylan esterases | [34] |

*Aspergillus awamori* IFO4033^{c} | 275 aa, 31 kDa | Wheat bran, α-naphthol acetate | Homology to lipases from *Geotrichum candidum* and *Candida cylindracea* | [78] |

*Saccharomyces cerevisiae* IFO2347 | 28 kDa, homodimer, T_{opt.} 25°C | Isoamyl acetate, isobutyl acetate | – | [79] |