• Open Access

Direct interaction between SNAP-23 and L-type Ca2+ channel


*Correspondence to: Bhanu P. JENA, Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E. Canfield, Detroit, MI, 48201, USA. Tel.: (313) 577-1532, Fax: (313) 993-4177; E-mail: bjena@med.wayne.eduhttp://www.med.wayne.edu/physiology/facultyprofile/jena


During secretion, membrane-bound secretory vesicles dock and fuse at the base of porosomes in the cell plasma membrane. Among other proteins, the porosome is composed of SNAREs and Ca2+ -channels. Ca2+-channels and SNAREs have been implicated in cell secretion. Several immunoprecipitation and binding studies suggest the physical interaction of the t-SNARE proteins, Syntaxin-1 and SNAP-25 with various Ca2+-channels. In this study, using yeast two-hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP-23 and a L-type Ca2+ -channel at the plasma membrane in pancreas.