• Open Access

Snapshots of mammary gland interstitial cells: methylene-blue vital staining and c-kit immunopositivity

Authors

  • L. M. Popescu,

    Corresponding author
    1. Department of Cellular and Molecular Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
    2. “Victor Babes” National Institute of Pathology, Bucharest, Romania
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  • F. Andrei,

    1. “Victor Babes” National Institute of Pathology, Bucharest, Romania
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  • M. E. Hinescu

    1. Department of Cellular and Molecular Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
    2. “Victor Babes” National Institute of Pathology, Bucharest, Romania
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*Correspondence to: L. M. Popescu, M.D., Ph.D.; E-mail: lpopescu@univermed-cdgm.ro

Abstract

We show here that methylene-blue supravital staining of specimens from normal mammary-gland reveals (selectively) interstitial (stromal) cells, with 2–3 long (20–80 μm), thin, moniliform processes. Such cells appear c-kit/CD117 positive, either by immunohistochemistry (IHC) or immunofluorescence (IF). Since these features (affinity for methylene blue, c-kit positivity, and characteristic processes) define archetypal interstitial cells of Cajal (ICC) in light microscopy, our results suggest the existence of Cajal-like cells in the interstitium of human normal mammary gland.

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