• Open Access

A new scoring system using multiple immunohistochemical markers for diagnosis of uterine smooth muscle tumors

Authors

  • Lea Rath-Wolfson,

    1. Department of Pathology, Hasharon Hospital, Rabin Medical Center; Petah-Tiva, Israel
    2. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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  • Yevgenia Rosenblat,

    1. Department of Pathology, Hasharon Hospital, Rabin Medical Center; Petah-Tiva, Israel
    2. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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  • Marisa Halpern,

    1. Department of Pathology, Hasharon Hospital, Rabin Medical Center; Petah-Tiva, Israel
    2. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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  • M. Herbert,

    1. Department of Pathology, Assaf Harofeh Hospital, Tsrifin, Israel
    2. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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  • I. Hammel,

    1. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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  • Rivka Gal,

    1. Department of Pathology, Hasharon Hospital, Rabin Medical Center; Petah-Tiva, Israel
    2. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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  • M. Leabu,

    1. Department of Cellular and Molecular Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
    2. Department of Cellular and Molecular Biology, “Victor Babes” Institute of Pathology and Biomedical Research, Bucharest, Romania
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  • Rumelia Koren

    Corresponding author
    1. Department of Pathology, Hasharon Hospital, Rabin Medical Center; Petah-Tiva, Israel
    2. Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
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* Correspondence to: Dr. Rumelia KOREN, Pathology Department, Hasharon Hospital, 7, KKL St. Petah-Tikva, Israel Tel.: 972-3-9372390 Fax: 972-3-9372349 E-mail: rumelia@isdnmail.co.il

Abstract

The diagnosis of uterine smooth muscle neoplasms by light microscopy is difficult. Multiple classification schemes have been proposed based on mitotic rate, nuclear atypia, and the presence or absence of necrosis. None of these classification systems has been entirely successful. This study was undertaken to evaluate the use of selected immunohistochemical and histochemical markers in differentiating these tumors, in addition to accepted morphologic criteria. Ten cases of each of the following: leiomyosarcomas (LMS), atypical leiomyomas (AL), cellular leiomyomas (CL) and usual leiomyomas (UL), were classically evaluated for histological diagnosis and were stained for Ki-67 (MIB-1), bcl-2 and p53 using monoclonal antibodies and the avidin-biotin peroxidase method, and argyrophilic nucleolar organizer region (AgNORs). The number of stained cells was counted in the most positively stained region in a 4 mm2 square cover glass mounted on each slide. The mean value was calculated for each group of tumors. The data for Ki-67 (MIB-1), bcl-2, p53 and AgNOR staining respectively, were significantly higher in LMS by comparison to UL, CL or AL. Because many singular cases had superimposed data being difficult to diagnose, a new scoring system for pathological evaluation was created. The results obtained by this scoring system suggest that immunohistochemical markers Ki-67 (MIB-1), bcl-2, p53 together with the AgNOR staining could be useful, by the scoring system, as an adjunct to the current accepted morphologic criteria in differentiating smooth muscle tumors of the uterus.

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