• Open Access

Gene transfer strategies in tissue engineering

Authors

  • Oliver Bleiziffer,

    1. Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany,
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  • Elof Eriksson,

    1. Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, USA
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  • Feng Yao,

    1. Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, USA
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  • Raymund E. Horch,

    1. Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany,
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  • Ulrich Kneser

    Corresponding author
    1. Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany,
      *Correspondence to: Ulrich KNESER, M.D.
      Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Krankenhausstr. 12, 91054
      Erlangen, Germany.
      Tel.: +49-9131-85-33277; Fax: +49-9131-85-39327
      E-mail: Ulrich.Kneser@chir.imed.uni-erlangen.de
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  • Guest Editor: R.E. Horch

*Correspondence to: Ulrich KNESER, M.D.
Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Krankenhausstr. 12, 91054
Erlangen, Germany.
Tel.: +49-9131-85-33277; Fax: +49-9131-85-39327
E-mail: Ulrich.Kneser@chir.imed.uni-erlangen.de

Abstract

  • • Introduction
  • • Methods of gene delivery
    • - Non-viral techniques
      • - Naked DNA
      • - Cationic liposomes
    • - Viral techniques
      • - Retrovirus
      • - Adenovirus
      • - Adeno-associated virus
      • - Herpes simplex virus
  • • Gene delivery from scaffolds for tissue engineering
  • • Gene therapy applications in tissue engineering
    • - Skin and wound healing
    • - Cartilage
    • - Bone
    • - Nerve
    • - Liver and endocrine pancreas
    • - Blood vessels
  • • Outlook

Abstract

Aiming for regeneration of severed or lost parts of the body, the combined application of gene therapy and tissue engineering has received much attention by regenerative medicine. Techniques of molecular biology can enhance the regenerative potential of a biomaterial by co-delivery of therapeutic genes, and several different strategies have been used to achieve that goal. Possibilities for application are many-fold and have been investigated to regenerate tissues such as skin, cartilage, bone, nerve, liver, pancreas and blood vessels. This review discusses advantages and problems encountered with the different gene delivery strategies as far as they relate to tissue engineering, analyses the positive aspects of polymeric gene delivery from matrices and discusses advances and future challenges of gene transfer strategies in selected tissues.

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