• Open Access

Human topoisomerase II-α is highly expressed in sinonasal-inverted papilloma, but not in inflammatory polyp

Authors

  • Tuvia Hadar,

    1. Department of Head and Neck Surgery, Rabin Medical Center, Petah Tikva, Israel
    2. Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
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    • #

      These authors contributed equally to this study.

  • Jacob Shvero,

    1. Department of Head and Neck Surgery, Rabin Medical Center, Petah Tikva, Israel
    2. Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
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    • #

      These authors contributed equally to this study.

  • Eitan Yaniv,

    1. Department of Head and Neck Surgery, Rabin Medical Center, Petah Tikva, Israel
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  • Itzhac Shvili,

    1. Department of Head and Neck Surgery, Rabin Medical Center, Petah Tikva, Israel
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  • Mircea Leabu,

    1. Department of Cellular and Molecular Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest Romania
    2. Division of Cellular and Molecular Biology, “Victor Babes” National Institute of Pathology, Bucharest Romania
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  • Rumelia Koren

    Corresponding author
    1. Department of Pathology, Rabin Medical Center, Petah Tikva, Israel
    2. Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
      *Correspondence to: Professor Rumelia KOREN, MD,
      Department of Pathology, Hasharon Hospital,
      Rabin Medical Center, Petah Tikva, Israel.
      Tel.: +972-3-9372390
      Fax: +972-3-9372349
      E-mail: rumeliak@clalit.org.il
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*Correspondence to: Professor Rumelia KOREN, MD,
Department of Pathology, Hasharon Hospital,
Rabin Medical Center, Petah Tikva, Israel.
Tel.: +972-3-9372390
Fax: +972-3-9372349
E-mail: rumeliak@clalit.org.il

Abstract

Sinonasal-inverted papilloma is a benign tumour with a high rate of recurrence, but possible malignant transformation. Therefore, inves tigation of predisposition to malignant transformation of sinonasal-inverted papilloma gives clinicians the opportunity for adequate trea ment. Topoisomerase II-α (topoII-α) and Ki67 are markers of cell proliferation in both normal and neoplastic tissues and its level o expression could be used as a predictive parameter. Our goal was to investigate by immunochemistry the expression level of topoII-in inverted papilloma, inflammatory nasal polyp and normal sinonasal epithelium and to compare it with expression level of Ki67. TopoI α nuclear immunostaining showed a differential positivity in the investigated cases. The topoII-α index was 30.6 ± 12.8 in inverte papilloma, 10.7 ± 6.6 in the adjacent epithelium of inverted papilloma, but only 2.3 ± 2.0 in the normal sinonasal epithelium. The di ferences in topoII-α expression between inverted papilloma and normal sinonasal epithelia were statistically significant. In inflammator nasal polyp group, topoII-α index was 2.4 ± 2.1, and the difference in the topoII-α index between inverted papilloma and inflammator polyp group was also statistically significant. Nuclear immunostaining for Ki67 followed a similar variation. The Ki67 index was 50.0 ± 20. in inverted papilloma, 9.0 ± 6.6 in the adjacent epithelium of inverted papilloma and 2.4 ± 0.9 in normal sinonasal epithelium. The di ferences in Ki67 expression between inverted papilloma and either adjacent or normal sinonasal epithelia were statistically significan Significant correlation coefficients were found between topoII-α and epithelial thickness (r = 0.70, P > 0.0001), and between Ki67 inde and epithelial thickness (r = 0.71, P> 0.0001). In the inflammatory nasal polyp group Ki67 index was 5.9 ± 3.4. The difference in th Ki67 index between inverted papilloma and inflammatory nasal polyp groups was statistically significant. Significant correlation coeff cient was found between topoII-α index and Ki67 index in inverted papilloma (r = 0.42, P > 0.05). These results suggest that the inverte papilloma contains a significantly higher cell population with proliferative activity by comparison with normal sinonasal and inflamma tory polyp epithelia, showing a significant correlation between topoII-α and Ki67 expression, and indicating that topoII-α could be a independent prognostic factor for a putative malignant transformation.

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