These authors contributed equally.
E2F1 represses β-catenin/TCF activity by direct up-regulation of Siah1
Article first published online: 9 JUL 2008
© 2008 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 13, Issue 8b, pages 1719–1727, August 2009
How to Cite
Xie, W., Jin, L., Mei, Y. and Wu, M. (2009), E2F1 represses β-catenin/TCF activity by direct up-regulation of Siah1. Journal of Cellular and Molecular Medicine, 13: 1719–1727. doi: 10.1111/j.1582-4934.2008.00423.x
- Issue published online: 26 NOV 2009
- Article first published online: 9 JUL 2008
- Received: November 13, 2007; Accepted: June 10, 2008
Transcription factor E2F1 is a key regulator of cell proliferation and apoptosis. Its activity is strictly controlled by the pRB/E2F pathway. In the majority of cancer cells, however, this pathway is frequently found deregulated, and the underlying mechanism involving transcriptional control by E2F1 has not yet been fully elucidated. Here we report the identification of two putative E2F1-binding sites located upstream from Siah1 transcription start site (+1). Chromatin immunoprecipitation assay reveals that transcription factor E2F1 is capable of binding to the putative sites, and luciferase reporter assay shows that E2F1 can activate transcription from the Siah1 promoter. Ectopic expression of E2F1 elevates the Siah1 level, hence suppressing the β-catenin/TCF activity. Consistently, knock-down of endogenous E2F1 by a shRNA strategy results in reduced expression of Siah1. Moreover, repression of β-catenin/TCF activity by E2F1 can be attenuated by shRNA-based repression of endogenous Siah1, implying that Siah1 is a bona fide E2F1 target gene, which at least partly, mediates the suppression of β-catenin/TCF signalling pathway.