These authors contributed equally to this work.
Mesenchymal stem cells induce a weak immune response in the rat striatum after allo or xenotransplantation
Article first published online: 26 NOV 2009
© 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 13, Issue 8b, pages 2547–2558, August 2009
How to Cite
Rossignol, J., Boyer, C., Thinard, R., Remy, S., Dugast, A.-S., Dubayle, D., Dey, N. D., Boeffard, F., Delecrin, J., Heymann, D., Vanhove, B., Anegon, I., Naveilhan, P., Dunbar, G. L. and Lescaudron, L. (2009), Mesenchymal stem cells induce a weak immune response in the rat striatum after allo or xenotransplantation. Journal of Cellular and Molecular Medicine, 13: 2547–2558. doi: 10.1111/j.1582-4934.2008.00657.x
- Issue published online: 26 NOV 2009
- Article first published online: 26 NOV 2009
- Received: August 4, 2008; Accepted: December 30, 2008
- mesenchymal stem cells;
- rat brain
Mesenchymal stem cells (MSCs) have attracted attention for their potential use in regenerative medicine such as brain transplantation. As MSCs are considered to be hypoimmunogenic, transplanted MSCs should not trigger a strong host inflammatory response. To verify this hypothesis, we studied the brain immune response after transplantation of human or rat MSCs into the rat striatum and MSC fate at days 5, 14, 21 and 63 after transplantation. Flow cytometry analysis indicated that both MSCs express CD90 and human leucocyte antigen (MHC) class I, but no MHC class II molecules. They do not express CD45 or CD34 antigens. However, MSC phenotype varies with passage number. Human MSCs have mRNAs for interleukin (IL)-6, IL-8, IL-12, tumour necrosis factor (TNF)-α and TGF-β1, whereas rat MSCs express IL-6-, IL-10-, IL-12- and TGF-β1-mRNAs. The quantification shows higher levels of mRNAs for the anti-inflammatory molecules IL-6 and TGF-β1 than for pro-inflammatory cytokines IL-8 and IL-12; ELISA analysis showed no IL-12 whereas TGF-β1 and IL-6 were detected. Transplant size did not significantly vary between 14 and 63 days after transplantation, indicating an absence of immune rejection of the grafts. Very few mast cells and moderate macrophage and microglial infiltrations, observed at day 5 remained stable until day 63 after transplantation in both rat and human MSC grafts. The observations of very few dendritic cells, T αβ-cells, and no T γδ-lymphocytes, all three being associated with Tp rejection in the brain, support the contention that MSCs are hypoimmunogenic. Our results suggest that MSCs are of great interest in regenerative medicine in a (xeno)transplantation setting.