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Fig. S1 Translocation of oestrogen receptors into the nucleus upon E2 treatment. Sub-cellular distribution of ERs consequent to treatment with 10 nM E2 or E2-BSA for 2 h is shown by immunofluorescence using an antibody, which recognizes both ER-α and ER-β. The panel stained green represents the ER-α/β staining (b), the blue staining represents nuclear staining with Hoecsht 33342 nuclear dye (c). The merge of images in panel b and c is shown in panel d. The panel "coloc mask" (e) represents the area within the cell showing colocalization of estrogen receptors with the nucleus. The value within the panel "coloc mask" represents the coefficient of colocalization of ER-α/β staining with the nuclear staining. Panel a represents phase contrast images. The bar represents 10 μm.

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