Clinical relevance and functional implications for human leucocyte antigen-g expression in non-small-cell lung cancer
Article first published online: 13 JUL 2009
© 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 14, Issue 9, pages 2318–2329, September 2010
Total views since August 2010: 369
How to Cite
Lin, A., Zhu, C.-C., Chen, H.-X., Chen, B.-F., Zhang, X., Zhang, J.-G., Wang, Q., Zhou, W.-J., Hu, W., Yang, H.-H., Xu, H.-H. and Yan, W.-H. (2010), Clinical relevance and functional implications for human leucocyte antigen-g expression in non-small-cell lung cancer. Journal of Cellular and Molecular Medicine, 14: 2318–2329. doi: 10.1111/j.1582-4934.2009.00858.x
- Issue published online: 13 JUL 2009
- Article first published online: 13 JUL 2009
- Received: February 5, 2009; Accepted: June 16, 2009
Fig. S1. Analysis of sHLA-G production in A549-G cells. (A) Analysis of sHLA-G in cell culture supernatants with ELISA. Culture supernantant from JAR-G5 (JAR cells transfected with HLA-G5) was used as an HLA-G5+ control. Medium Con., Medium controls. The dash indicates the background level. sHLA-G was undetectable in A549-G, A549 cell culture supernantants. (B) Western blot analysis of sHLA-G expression. The analysis was performed with the sHLA-G mAb 5A6G7 (1:1000). M, molecular weight ladder; JAR-G5 lysates were used as HLA-G5+ control. Isotype, a mouse IgG1 isotype antibody (1:1000) was used as internal control for JAR-G5 lysates. (C) Analysis of sHLA-G with immunocytochemistry. The analysis was performed with the sHLA-G mAb 5A6G7 (1:1000). JAR and JAR-G5 cells were used as HLA-G5- and HLA-G5+ controls, respectively. No intracellular sHLA-G staining was detected in A549-G cells.
Fig. S2. Flow cytometry analysis of HLA-G expression in peripheral blood CD45+ nucleated cells. (A) An IgG isotype was used as an internal control. (B) Analysis of cell surface HLA-G expression in CD45+ peripheral blood cells with mAb FITC-MEM-G/09. (C), (D) Analysis of intracellular sHLA-G expression in CD45+ peripheral blood cells with FITC-2A12.
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