Microselection – affinity selecting antibodies against a single Rare cell in a heterogeneous population
Article first published online: 14 SEP 2009
Journal of Cellular and Molecular Medicine
- Issue published online: 14 SEP 2009
- Article first published online: 14 SEP 2009
- Accepted manuscript online: 4 AUG 2010 11:57AM EST
- Received date: 21-Mar-09, Revised date: 3-Aug-09, Accepted date: 11-Aug-09
- Single cell selection;
- phage display;
- antibody selection;
- UV irradiation;
- Rare cell detection;
Rare cells not normally present in the peripheral bloodstream, such as circulating tumor cells, have potential applications for development of non-invasive methods for diagnostics or follow up. Obtaining these cells however require some means of discrimination, achievable by cell type specific antibodies.
Here we have generated a microselection method allowing antibody selection, by phage display, targeting a single cell in a heterogeneous population. One K562 cell (female origin) was positioned on glass-slide among millions of lymphocytes from male donor, identifying the K562 cell by FISH (XX). Several single cell selections were performed on such individual slides. The phage particles bound to the target cell is protected by a minute disc, while inactivating all remaining phage by UV-irradiation; leaving only the phage bound to the target cell viable. We hereby retrieved up to eight antibodies per single cell selection, including three highly K562 cell type specific.