These authors contributed equally to this work.
Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model
Article first published online: 14 MAY 2010
© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 15, Issue 6, pages 1310–1318, June 2011
How to Cite
Ou, L., Li, W., Zhang, Y., Wang, W., Liu, J., Sorg, H., Furlani, D., Gäbel, R., Mark, P., Klopsch, C., Wang, L., Lützow, K., Lendlein, A., Wagner, K., Klee, D., Liebold, A., Li, R.-K., Kong, D., Steinhoff, G. and Ma, N. (2011), Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model. Journal of Cellular and Molecular Medicine, 15: 1310–1318. doi: 10.1111/j.1582-4934.2010.01086.x
- Issue published online: 29 MAY 2011
- Article first published online: 14 MAY 2010
- Accepted manuscript online: 4 AUG 2010 11:58AM EST
- Received: November 12, 2009; Accepted: May 10, 2010
- cardiac regeneration;
- stem and progenitor cells
Matrigel promotes angiogenesis in the myocardium from ischemic injury and prevents remodelling of the left ventricle. We assessed the therapeutic efficacy of intracardiac matrigel injection and matrigel-mediated stem cell homing in a rat myocardial infarction (MI) model. Following MI, matrigel (250 μl) or phosphate-buffered solution (PBS) was delivered by intracardiac injection. Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure–volume loops after 4 weeks. There is no significant difference in infarct size between MI-matrigel (MI-M; 21.48 ± 1.49%, n= 10) and MI-PBS hearts (20.98 ± 1.25%, n= 10). The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n= 10) compared with MI-PBS (0.62 ± 0.02 mm, n= 10). MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n= 6) than MI-PBS hearts. c-Kit+ stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit+ cells per HPF [630×], n= 5, P < 0.05) and CD34+ cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34+ cells per HPF [630×], n= 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n= 5, P < 0.05). Intracardiac matrigel injection restores myocardial functions following MI, which may attribute to the improved recruitment of CD34+ and c-Kit+ stem cells.