• Open Access

Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model

Authors

  • Lailiang Ou,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
    2. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, China
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    • These authors contributed equally to this work.

  • Wenzhong Li,

    Corresponding author
    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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    • These authors contributed equally to this work.

  • Yue Zhang,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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    • These authors contributed equally to this work.

  • Weiwei Wang,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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    • These authors contributed equally to this work.

  • Jun Liu,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Heiko Sorg,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Dario Furlani,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Ralf Gäbel,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Peter Mark,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Christian Klopsch,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Liang Wang,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Karola Lützow,

    1. Institute of Polymer Research, GKSS Research Center, Geesthacht, Germany
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  • Andreas Lendlein,

    1. Institute of Polymer Research, GKSS Research Center, Geesthacht, Germany
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  • Klaus Wagner,

    1. Department of Anesthesia, Klinikum Südstadt Rostock, Rostock, Germany
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  • Doris Klee,

    1. Institute of Technical and Macromolecular Chemistry Aachen, Aachen, Germany
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  • Andreas Liebold,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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  • Ren-Ke Li,

    1. Division of Cardiovasculary Surgery, Toronto General Hospital and the University of Toronto, Toronto, Canada
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  • Deling Kong,

    1. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, China
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  • Gustav Steinhoff,

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
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    • These authors contributed equally to this work.

  • Nan Ma

    1. Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany
    2. Institute of Polymer Research, GKSS Research Center, Geesthacht, Germany
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    • These authors contributed equally to this work.


Correspondence to: Dr. Wenzhong LI, Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Schillingallee 69, 18057 Rostock, Germany.
Tel.: +49-381-494 6105
Fax: +49-381-494 6214
E-mail: wenzhong.li@med.uni-rostock.de

Abstract

Matrigel promotes angiogenesis in the myocardium from ischemic injury and prevents remodelling of the left ventricle. We assessed the therapeutic efficacy of intracardiac matrigel injection and matrigel-mediated stem cell homing in a rat myocardial infarction (MI) model. Following MI, matrigel (250 μl) or phosphate-buffered solution (PBS) was delivered by intracardiac injection. Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure–volume loops after 4 weeks. There is no significant difference in infarct size between MI-matrigel (MI-M; 21.48 ± 1.49%, n= 10) and MI-PBS hearts (20.98 ± 1.25%, n= 10). The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n= 10) compared with MI-PBS (0.62 ± 0.02 mm, n= 10). MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n= 6) than MI-PBS hearts. c-Kit+ stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit+ cells per HPF [630×], n= 5, P < 0.05) and CD34+ cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34+ cells per HPF [630×], n= 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n= 5, P < 0.05). Intracardiac matrigel injection restores myocardial functions following MI, which may attribute to the improved recruitment of CD34+ and c-Kit+ stem cells.

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