• Open Access

Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers

Authors

  • Hadas Sherman,

    1. Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
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    • These authors contributed equally.

  • Idan Frumin,

    1. Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
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    • These authors contributed equally.

  • Roee Gutman,

    1. Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
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  • Nava Chapnik,

    1. Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
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  • Axel Lorentz,

    1. Department of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany
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  • Jenny Meylan,

    1. Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland
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  • Johannes le Coutre,

    1. Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland
    2. The University of Tokyo, Organization for Interdisciplinary Research Projects, Yayoi, Bunkyo-ku, Tokyo, Japan
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  • Oren Froy

    Corresponding author
    1. Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
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Oren FROY, Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel. Tel.: 972–8-948–9746 Fax: 972-8-936-3208 E-mail: froy@agri.huji.ac.il

Abstract

The circadian clock in peripheral tissues can be entrained by restricted feeding (RF), a regimen that restricts the duration of food availability with no calorie restriction (CR). However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR. We measured circadian expression of clock genes, disease marker genes, metabolic factors and inflammatory and allergy markers in mouse serum, liver, jejunum and white adipose tissue (WAT) after long-term RF of 4 months. We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF. In addition, average daily levels of some disease and inflammatory markers were reduced under RF, including liver Il-6 mRNA, tumour necrosis factor (TNF)-α and nuclear factor κB (NF-κB) protein; jejunum Arginase, Afp, Gadd45β, Il-1α and Il-1β mRNA, and interleukin (IL)-6 and TNF-α protein and WAT Il-6, Il-1β, Tnfα and Nfκb mRNA. In contrast, the anti-inflammatory cytokine Il-10 mRNA increased in the liver and jejunum. Our results suggest that RF may share some benefits with those of CR. As RF is a less harsh regimen to follow than CR, the data suggest it could be proposed for individuals seeking to improve their health.

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