These authors contributed equally to this work.
Mature endothelium and neurons are simultaneously derived from embryonic stem cells by 2D in vitro culture system
Version of Record online: 26 SEP 2011
© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 15, Issue 10, pages 2200–2215, October 2011
How to Cite
Noghero, A., Arese, M., Bussolino, F. and Gualandris, A. (2011), Mature endothelium and neurons are simultaneously derived from embryonic stem cells by 2D in vitro culture system. Journal of Cellular and Molecular Medicine, 15: 2200–2215. doi: 10.1111/j.1582-4934.2010.01209.x
- Issue online: 26 SEP 2011
- Version of Record online: 26 SEP 2011
- Accepted manuscript online: 10 NOV 2010 10:11AM EST
- Received: May 20, 2010; Accepted: November 2, 2010
Fig. S1 Quantification by FACS analysis of the neurons differentiated within ES-PA6 co-cultures.
Fig. S2 Cytofluorimetric absolute cells count of ES-derived cells and PA6 cells.
Fig. S3 biochemical detection and quantification of Oct4 protein in ES-PA6 co-cultures.
Fig. S4 analysis of gene expression upon FGF2 or BMP4 stimulation.
Fig. S5 characterization by immunofluorescence analysis of ES-PA6 cocultures for the presence of glia, endoderm, pancreatic and smooth muscle cells.
Fig. S6 Gene expression analysis in pure PA6 cell cultures stimulated with FGF2.
Fig. S7 2D in vitro differentiation of Flk1-/- and Tal1-/- ES cell lines.
Table S1 List of shRNA MISSION clones used to stably knock-down the expression of Flk1, Ace, Tal1 and T.
Table S2 list of the genes used to customize the Taqman Low Density Array.
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