• Open Access

Catalase activity prevents exercise-induced up-regulation of vasoprotective proteins in venous tissue

Authors

  • Vu Thao-Vi Dao,

    1. Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Melanie Floeren,

    1. Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Stephanie Kumpf,

    1. Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Charlotte Both,

    1. Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Bärbel Peter,

    1. Animal facilities, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Vera Balz,

    1. Institute for Transplantationdiagnostic and Cell Therapeutics, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Tatsiana Suvorava,

    1. Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
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  • Georg Kojda

    Corresponding author
    1. Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany
      Georg KOJDA, Institut fuer Pharmakologie und klinische Pharmakologie, Heinrich-Heine-Universitaet, Moorenstr. 5, 40225 Duesseldorf, Germany. Tel.: +49-211-81-12518 Fax: +49-211-81-14781 E-mail: kojda@uni-duesseldorf.de
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Georg KOJDA, Institut fuer Pharmakologie und klinische Pharmakologie, Heinrich-Heine-Universitaet, Moorenstr. 5, 40225 Duesseldorf, Germany. Tel.: +49-211-81-12518 Fax: +49-211-81-14781 E-mail: kojda@uni-duesseldorf.de

Abstract

Physical activity induces favourable changes of arterial gene expression and protein activity, although little is known about its effect in venous tissue. Although our understanding of the initiating molecular signals is still incomplete, increased expression of endothelial nitric oxide synthase (eNOS) is considered a key event. This study sought to investigate the effects of two different training protocols on the expression of eNOS and extracellular superoxide dismutase (ecSOD) in venous and lung tissue and to evaluate the underlying molecular mechanisms. C57Bl/6 mice underwent voluntary exercise or forced physical activity. Changes of vascular mRNA and protein levels and activity of eNOS, ecSOD and catalase were determined in aorta, heart, lung and vena cava. Both training protocols similarly increased relative heart weight and resulted in up-regulation of aortic and myocardial eNOS. In striking contrast, eNOS expression in vena cava and lung remained unchanged. Likewise, exercise up-regulated ecSOD in the aorta and in left ventricular tissue but remained unchanged in lung tissue. Catalase expression in lung tissue and vena cava of exercised mice exceeded that in aorta by 6.9- and 10-fold, respectively, suggesting a lack of stimulatory effects of hydrogen peroxide. In accordance, treatment of mice with the catalase inhibitor aminotriazole for 6 weeks resulted in significant up-regulation of eNOS and ecSOD in vena cava. These data suggest that physiological venous catalase activity prevents exercise-induced up-regulation of eNOS and ecSOD. Furthermore, therapeutic inhibition of vascular catalase might improve pulmonary rehabilitation.

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