Fig. S1 FACS analysis of surface CXCR4 on BMC. After calcium treatment, both the number of cells expressing surface CXCR4 and the amount of CXCR4 on the surface are increased. The FACS pictures show that more cells above the base line indicating more CXCR4+ cells, and that higher intensity of fluorescence (higher above the line) indicating increased amount of CXCR4 on surface.

Fig. S2 Calcium influx into BMC. Calcium influx into cells was measured by flow cytometry with Fluo-4/AM staining. Arrows indicate the time when PBS or CaCl2 were added. The FACS data were converted into line graphs in Figure 1F.

Fig. S3 Viability and apoptosis of BMCs. BMCs from young (white bar) and old (black bar) mice were cultured under hypoxia (0.5% O2) in serum-free DMEM supplemented with or without SDF-1α for 20 hrs. The apoptosis and necrosis cells were detected by annexin V and PI staining and thereafter flow cytometry analysis. The unhealthy cells represent the cells that were positive for either staining. *P < 0.05; n = 3.

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