Screening of deoxyribozyme with high reversal efficiency against multidrug resistance in breast carcinoma cells
Article first published online: 26 SEP 2011
© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 15, Issue 10, pages 2130–2138, October 2011
How to Cite
Gao, P., Wei, J.-M., Li, P.-Y., Zhang, C.-J., Jian, W.-C., Zhang, Y.-H., Xing, A.-Y. and Zhou, G.-Y. (2011), Screening of deoxyribozyme with high reversal efficiency against multidrug resistance in breast carcinoma cells. Journal of Cellular and Molecular Medicine, 15: 2130–2138. doi: 10.1111/j.1582-4934.2010.01240.x
- Issue published online: 26 SEP 2011
- Article first published online: 26 SEP 2011
- Accepted manuscript online: 14 DEC 2010 07:43AM EST
- Received: June 24, 2010; Accepted: November 2, 2010
Fig. S1 The secondary structure of 5′-region of MDR1 mRNA is shown and the twelve R-Y dinucleotides on the surface of the MDR1 mRNA secondary structure were selected as targets for DRzs.
Fig. S2 (A) Anti-miR-27a inhibitor and ASODN could only suppress Pgp expression at concentration of 5 μg/ml or above (P < 0.05). However, DRz 3 could suppress it at concentrations of 0.5 μg/ml or above, in a dose-dependent response in MDA/ADR cells. (B) During the continuous observations of DRz at 5 μg/ml, the inhibitory effect of DRz 3 on Pgp expression was better and longer than that for the other two groups in MDA/ADR cells (P < 0.05).
Fig. S3 Rh123 retention showed that intracellular Rh123 in cells treated with DRz 3 was significantly higher than that of the other two groups in MDA-7/ADR cells.
Table S1 Fold change of MDR1 mRNA and chemosensitivity assay in MDA/ADR cells transfected with DRzs
Table S2 Cell toxicity of DRz 3, ASODN, ribozyme and anti-miR-27a inhibitor
Table S3 Evaluation of chemosensitivity in the transfected MDA/ADR cells
|JCMM_1240_sm_suppmat.doc||291K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.