Evaluation of exon-skipping strategies for Duchenne muscular dystrophy utilizing dystrophin-deficient zebrafish
Version of Record online: 28 NOV 2011
© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
Journal of Cellular and Molecular Medicine
Volume 15, Issue 12, pages 2643–2651, December 2011
How to Cite
Berger, J., Berger, S., Jacoby, A. S., Wilton, S. D. and Currie, P. D. (2011), Evaluation of exon-skipping strategies for Duchenne muscular dystrophy utilizing dystrophin-deficient zebrafish. Journal of Cellular and Molecular Medicine, 15: 2643–2651. doi: 10.1111/j.1582-4934.2011.01260.x
- Issue online: 28 NOV 2011
- Version of Record online: 28 NOV 2011
- Accepted manuscript online: 20 JAN 2011 12:06PM EST
- Received: August 10, 2010; Accepted: January 10, 2011
Fig. S1dmd allele identification. The following primer pairs amplify the dystrophin locus, characteristic for each allele, followed by restriction digestion with the designated enzyme. Resulting DNA band patterns are as depicted. dmdpc1: pc1F 5'-ctgcagtctccatccaagt-3' and pc1R 5'-accaagtcctggaggacctgagggccagggatcga-3', ClaI. dmdpc2: pc2F 5'-aatgcctgtaaacaaatgtgtctgt-3' and pc2R 5'-ccttgccatgttaacccaaa-3', BsaXI. dmdta222a: ta222aF 5'-catacccaaggtttcaaagca-3' and ta222aR 5'-gttaagggagtgcactcgagtgaagccacgttttt-3', DraI. dmdtm90c: tm90cF 5'-actgcagaacatgtataaggactccagcgctt-3' and tm90cR 5'-aattaccttgacatctgcattttggcc-3', DdeI.
Fig. S2 Comparison of human and zebrafish dystrophin. Numbered boxes represent exons of the human dystrophin isoform Dp427m with amino acid numbers indicated. The conservation of each exon in comparison to the zebrafish dystrophin sequence is written inside each box. Blue boxes represent exons that have the same number of amino acids in human and zebrafish and yellow ones have a different number of amino acids, as indicated below each yellow box.
Fig. S3 Variation of exon skipping in individual larvae. At 3 dpf, the exon-skipping efficiency was analysed in individual larvae administered with an equal mixture of Z32E(+133+157) and Z32E(+83+107) at a concentration of 12 μM each. The variation in exon-skipping efficiency is relatively low, allowing pooling of two larvae for subsequent analysis in further experiments.
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