Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures

  1. Hui-Ting Chen1,2,†,
  2. Mon-Juan Lee2,10,†,
  3. Chung-Hwan Chen2,3,6,7,
  4. Shu-Chun Chuang2,
  5. Li-Fu Chang2,
  6. Mei-Ling Ho2,3,4,5,6,
  7. Shao-Hung Hung2,11,
  8. Yin-Chih Fu2,3,6,7,
  9. Yan-Hsiung Wang2,8,
  10. Hsin-I Wang2,
  11. Gwo-Jaw Wang2,7,12,14,
  12. Lin Kang13,
  13. Je-Ken Chang2,3,7,8,*

DOI: 10.1111/j.1582-4934.2011.01335.x

Additional Information

Author Information

  1. 1

    Department of Fragrance and Cosmetic Science

  2. 2

    Orthopaedic Research Center

  3. 3

    Faculty of Medicine, Departments of Orthopaedics

  4. 4

    Faculty of Medicine, Physiology

  5. 5

    Graduate Institute of Physiology and Molecular Medicine, College of Medicine

  6. 6

    Graduate Institute of Medicine, College of Medicine

  7. 7

    Department of Orthopaedics, Kaohsiung Medical University Hospital

  8. 8

    School of Dentistry, College of Dental Medicine

  9. 10

    Department of BioScience Technology, Chang Jung Christian University, Tainan, Taiwan

  10. 11

    Department of Orthopedic Surgery, Fooyin University Hospital, Tungkang Chen, Ping-Tung County, Taiwan.

  11. 12

    Department of Orthopaedics, National Cheng Kung University Medical College and Hospital, Tainan, Taiwan

  12. 13

    Department of Obstetrics and Gynecology, National Cheng Kung University Medical College and Hospital, Tainan, Taiwan

  13. 14

    Department of Orthopedic Surgery, University of Virginia, Virginia, U.S.A.

  1. †

    These authors contributed equally to this manuscript.

* Correspondence to: Je-Ken Chang, Department of Orthopaedics, School of Medicine, Kaohsiung Medical University and Kaohsiung Medical University Hospital, No. 100, Shih-Chuan 1st Rd., Kaohsiung 807, Taiwan. Tel.: +886 7 3121101 ext. 5751 Fax: +886 7 3119544/3219452 E-mail: jkchang@cc.kmu.edu.tw

Publication History

  1. Accepted manuscript online: 5 MAY 2011 09:48AM EST
  2. Received: May 20, 2010, Accepted: April 27, 2011

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Keywords:

  • adipose tissue-derived stem cell (ADSC);
  • aging;
  • bone marrow-derived mesenchymal stem cell (BMSC);
  • osteogenic differentiation;
  • osteoporosis;
  • p21;
  • proliferation;
  • senescence-associated β-galactosidase

Abstract

Aging has less effect on adipose-derived mesenchymal stem cells (ADSCs) than on bone marrow-derived mesenchymal stem cells (BMSCs), but whether the fact holds true in stem cells from elderly patients with osteoporotic fractures is unknown. In this study, ADSCs and BMSCs of the same donor were harvested and divided into two age groups. Group A consisted of 14 young patients (36.4 ± 11.8 years old), and group B consisted of 8 elderly patients (71.4 ± 3.6 years old) with osteoporotic fractures. We found that the doubling time of ADSCs from both age groups was maintained below 70 hrs, while that of BMSCs increased significantly with the number of passage. When ADSCs and BMSCs from the same patient was compared, there was a significant increase in the doubling time of BMSCs in each individual from passages 3 to 6. Upon osteogenic induction, the level of matrix mineralization of ADSCs from group B was comparable to that of ADSCs from group A, while BMSCs from group B produced least amount of mineral deposits and had a lower expression level of osteogenic genes. The p21 gene expression and senescence-associated β-galactosidase activity were lower in ADSCs compared to BMSCs, which may be partly responsible for the greater proliferation and differentiation potential of ADSCs. It is concluded that the proliferation and osteogenic differentiation of ADSCs were less affected by age and multiple passage than BMSCs, suggesting that ADSCs may become a potentially effective therapeutic option for cell-based therapy, especially in elderly patients with osteoporosis.

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