• Open Access

Repertoire of endothelial progenitor cells mobilized by femoral artery ligation: a nonhuman primate study

Authors

  • Qiang Shi,

    Corresponding author
    1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA
      Qiang SHI, Southwest National Primate Research Center at the Texas Biomedical Research Institute, P.O. Box 760549, San Antonio, TX 78245-0549, USA. Tel.: (210) 258-9703 Fax: (210) 258-9796 E-mail: qshi@txbiomedgenetics.org
    Search for more papers by this author
  • Laura A. Cox,

    1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA
    2. Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX, USA
    Search for more papers by this author
  • Vida Hodara,

    1. Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
    Search for more papers by this author
  • Xing Li Wang,

    1. Cardiothoracic Research Laboratory, Texas Heart Institute, Baylor College of Medicine, Houston, TX, USA
    Search for more papers by this author
  • John L. VandeBerg

    1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA
    2. Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX, USA
    Search for more papers by this author

Qiang SHI, Southwest National Primate Research Center at the Texas Biomedical Research Institute, P.O. Box 760549, San Antonio, TX 78245-0549, USA. Tel.: (210) 258-9703 Fax: (210) 258-9796 E-mail: qshi@txbiomedgenetics.org

Abstract

To determine in the baboon model the identities and functional characteristics of endothelial progenitor cells (EPCs) mobilized in response to artery ligation, we collected peripheral blood mononuclear cells (PBMNCs) before and 3 days after a segment of femoral artery was removed. Our goal was to find EPC subpopulations with highly regenerative capacity. We identified 12 subpopulations of putative EPCs that were altered >1.75-fold; two subpopulations (CD146+/CD54–/CD45– at 6.63-fold, and CD146+/UEA-1–/CD45– at 12.21-fold) were dramatically elevated. To investigate the regenerative capacity of putative EPCs, we devised a new assay that maximally resembled their in vivo scenario, we purified CD34+ and CD146+ cells and co-cultured them with basal and mobilized PBMNCs; both cell types took up Dil-LDL, but purified CD146+ cells exhibited accelerated differentiation by increasing expression of CD31 and CD144, and by exhibiting more active cord-like structure formation by comparison to the CD34+ subpopulation in a co-culture with mobilized PBMNCs. We demonstrate that ischaemia due to vascular ligation mobilizes multiple types of cells with distinct roles. Baboon CD146+ cells exhibit higher reparative capacity than CD34+ cells, and thus are a potential source for therapeutic application.

Ancillary