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jcmm1609-sup-0001-FigureS1.docWord document665KFig. S1. Paraffin embedded cross-sections of the human resistance gastro-omental arteries stained with haematoxylin and eosin. (A, B) showing cross-section of the typical arteries used in the experiments from two different patients. The arteries normally displayed between 3 and 6 layers of smooth muscle cells in medial layer and presence of some connective tissue in the adventitial layer. (Bi) shows enlarged fragment of the artery depicted by dashed rectangle in (B).
jcmm1609-sup-0002-FigureS2.docWord document157KFig. S2. Application of 10 mmol/l caffeine results in contraction of VSMC but not VIC from human gastro-omental artery. Cells were pre-loaded with calcium indicator Fluor-3am. (A) transmitted light image. (B) fluorescence images of Fluo-3 loaded cells (a) before, (b) at the moment and (c) 10 sec. after 10 mmol/l caffeine application. Red and yellow colours indicate increase in intracellular calcium. Please note the increase in intracellular calcium in response to agonist in both VSMCs and VICs. Please also note the contraction of VSMC (white arrow). Horizontal bar corresponds 20 μm.
jcmm1609-sup-0003-FigureS3.docWord document356KFig. S3. Freshly dispersed VSMC from human gastro-omental arteries does not display any significant changes in shape similar to those recorded in VICs during same period of time. Live imaging performed for 20 min. at room temperature. Transmitted light images taken with 10 min. interval. Horizontal bar corresponds 10 μm.
jcmm1609-sup-0004-FigureS4.docxWord document359KFig. S4. Absence of rigid cytoskeleton in VICs compared to VSMCs. (A) VIC easily passes into a glass pipette with inner diameter less than half of the size of the cell. (B) similar in size VSMC blocks the pipette. Inset (A1i) shows that orifice of the pipette (dashed oval) is less than 3 μm. A moderate negative pressure is applied to the pipette. The horizontal bar corresponds 10 μm.
jcmm1609-sup-0005-VideoS1.wmvvideo/wmv3664KVideo S1. Separate collection of isolated VICs and SMCs.
jcmm1609-sup-0006-VideoS2.wmvvideo/wmv1700KVideo S2. Active change in shape of VIC.
jcmm1609-sup-0007-VideoS3.wmvvideo/wmv4987KVideo S3. Absence of rigid cytoskeleton in VIC.

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