• Open Access

BDNF-mediated migration of cardiac microvascular endothelial cells is impaired during ageing

Authors

  • Liang Cao,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    4. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
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  • Liang Zhang,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    4. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
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  • Siyun Chen,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
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  • Ziqiang Yuan,

    1. Department of Molecular Genetics, Albert Einstein College of Medicine, New York, NY, USA
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  • Shaokun Liu,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
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  • Xiaotao Shen,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    4. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
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  • Xin Zheng,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    4. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
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  • Xufeng Qi,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    4. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
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  • Kenneth K.H. Lee,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. Stem cell and Regeneration TRP, School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, Hong Kong
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  • John Yeuk-Hon Chan,

    1. Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
    2. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    3. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    4. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
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  • Dongqing Cai

    Corresponding author
    1. Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou, China
    2. International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China
    3. Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou, China
    • Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China
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Correspondence to: Dongqing CAI, Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou 510632, China.

E-mail: tdongbme@jnu.edu.cn

Abstract

This study indicates that brain-derived neurotrophic factor (BDNF) can promote young cardiac microvascular endothelial cells (CMECs) to migrate via the activation of the BDNF-TrkB-FL-PI3K/Akt pathway, which may benefit angiogenesis after myocardial infarction (MI). However, the ageing of CMECs led to changes in the expression of receptor Trk isoforms in that among the three isoforms (TrkB-FL, TrkB-T1 and TrkB-T2), only one of its truncated isoforms, TrkB-T1, continued to be expressed, which leads to the dysfunction of its ligand, a decrease in the migration of CMECs and increased injury in ageing hearts. This shift in receptor isoforms in aged CMECs, together with changes in the ageing microenvironment, might predispose ageing hearts to decreased angiogenic potential and increased cardiac pathology.

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