PROTEIN SYNTHESIS IN MUSCLE CULTURES FROM PATIENTS WITH DUCHENNE MUSCULAR DYSTROPHY
Calcium and A23187 ionophore dependent changes
Article first published online: 29 JAN 2009
1976 Blackwell Munksgaard
Acta Neurologica Scandinavica
Volume 54, Issue 3, pages 241–247, October 1976
How to Cite
Ionasescu, V., Zellweger, H., Ionasescu, R., Lara-Braud, C. and Cancilla, P. A. (1976), PROTEIN SYNTHESIS IN MUSCLE CULTURES FROM PATIENTS WITH DUCHENNE MUSCULAR DYSTROPHY. Acta Neurologica Scandinavica, 54: 241–247. doi: 10.1111/j.1600-0404.1976.tb04800.x
- Issue published online: 29 JAN 2009
- Article first published online: 29 JAN 2009
- Received March 5. 1976.
Muscle samples for cultures were obtained from the quadriceps by open biopsy under local anesthesia in five patients with early stage of Duchenne muscular dystrophy (DMD) and 10 controls. Primary cultures were grown in Eagle's Minimum Essential Medium (MEM) with 20 per cent fetal calf serum. After 4 weeks, cells were trypsinized, counted, sub-cultured for 5 days in MEM with 5 per cent horse serum and finally incubated for 4 h with (3H) leucine. Total protein synthesis showed a significant decrease (half of control values) only in muscle cultures from patients with DMD. Addition of calcium chloride alone or with A23187 ionophore normalized this defect in protein synthesis. By contrast, myosin heavy chain synthesis was measured and found normal in all patients.