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Rituximab in a patient with multiple sclerosis – effect on B cells, plasma cells and intrathecal IgG synthesis

Authors


Hela-F. Petereit, Department of Neurology, Heilig Geist Krankenhaus, Graseggerstr. 105, 50737 Köln, Germany
Tel.: +49 221 7491 1024
Fax: +49 221 7491 1067
e-mail: petereit@hgk-koeln.de

Abstract

Objective –  To study the time course of immunoglobulin, B and plasma cells in the blood and cerebrospinal fluid (CSF) before and during rituximab treatment in a patient with severe relapsing–remitting multiple sclerosis (MS) in relation to clinical and MRI findings.

Methods –  Immunoglobulins in the CSF were measured by nephelometry and detected by isoelectrical focussing. CSF and blood cell subtypes from seven time points were analysed by flow cytometry.

Results –  Treatment with rituximab induced a dramatic and sustained improvement in clinical and MRI findings over a follow-up period of 20 months. By contrast, the initially completely suppressed B and plasma cells in both the blood and CSF reappeared after 5 and 10 months, CSF cells being the first to reappear. Interestingly, intrathecal IgG synthesis persisted throughout the study period.

Discussion –  Although highly effective in this case, the clinical effect in larger series and the mechanism of rituximab in MS deserves further evaluation.

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