Analysis of CD177 neutrophil expression in β-thalassemia patients
Version of Record online: 22 JUL 2011
© 2011 The Authors. APMIS © 2011 APMIS
Volume 119, Issue 10, pages 674–680, October 2011
How to Cite
MONTASER, L. M., EL-RASHIDI, F. H., ESSA, E. S. and AZAB, S. M. (2011), Analysis of CD177 neutrophil expression in β-thalassemia patients. APMIS, 119: 674–680. doi: 10.1111/j.1600-0463.2011.02755.x
- Issue online: 14 SEP 2011
- Version of Record online: 22 JUL 2011
- Received 6 November 2010. Accepted 15 March 2011
- flow cytometry
Montaser LM, El-Rashidi FH, Essa ES, Azab SM. Analysis of CD177 neutrophil expression in β-thalassemia patients. APMIS 2011; 119: 674–80.
Ineffective erythropoiesis plays a well established role in the pathophysiology of disease expression in β-thalassemia major and intermedia. CD177 expression was investigated in different clinical conditions. The study aimed to analyze neutrophil expression of CD177 in β-thalassemia patients and its correlation with serum soluble transferrin receptor (s-TfR) concentration as a marker for the extent of erythropoiesis and hence disease severity in these patients. Flow cytometric analysis of neutrophil CD177 expression and enzyme immunoassay measurement of serum s-TfR concentration were assessed in 45 β-thalassemia patients of whom 36 had β-thalassemia major and nine had β-thalassemia intermedia. They were also assessed in 21 age- and gender-matched control children. Neutrophil mean fluorescence intensity ratio (MFIR) of CD177 expression was significantly higher in patients than in controls (p < 0.001). There was highly significant increase in serum s-TfR concentration in β-thalassemia patients than in controls (p < 0.001). There was a highly significant positive correlation between MFIR of CD177 expression and serum s-TfR concentration in β-thalassemia patients (r = 0.59, p < 0.001). Elevated CD177 expression is not only a specific feature of polycythemia rubra vera (PV) but may be also an indicator of increased erythropoietic activity in thalassemia syndromes.