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Biofilm formation by bacteria isolated from upper respiratory tract before and after adenotonsillectomy

Authors

  • LORENZO DRAGO,

    1. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Institute, Milan, Italy
    2. Laboratory of Clinical Microbiology, Department of Clinical Science, University of Milan, Italy
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  • ELENA DE VECCHI,

    1. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Institute, Milan, Italy
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  • SARA TORRETTA,

    1. Department of Specialistic Surgical Sciences, “Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico”, University of Milan, Milan, Italy
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  • ROBERTO MATTINA,

    1. Department of Public Health, Microbiology and Virology, University of Milan, Italy
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  • PAOLA MARCHISIO,

    1. Department of Maternal and Paediatric Sciences, “Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico”, University of Milan, Milan, Italy
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  • LORENZO PIGNATARO

    1. Department of Specialistic Surgical Sciences, “Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico”, University of Milan, Milan, Italy
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L Drago, Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute,Via R. Galeazzi 4, 20161 Milan, Italy. e-mail: lorenzo.drago@unimi.it

Abstract

Drago L, De Vecchi E, Torretta S, Mattina R, Marchisio P, Pignataro L. Biofilm formation by bacteria isolated from upper respiratory tract before and after adenotonsillectomy. APMIS 2012; 120: 410–6.

Failure of antibiotics to eradicate the microbial pathogens primarily responsible for otorhinolaryngological diseases has led to the hypothesis that these microorganisms may be structured in a biolfilm. Aim of the study was to evaluate the ability to produce biofilm among bacteria isolated from tonsils and/or adenoids and nasopharynx. Biopsies and swabs were collected during surgery and after 3 and 6 months in 32 children undergoing adenoidectomy and/or tonsillectomy. Production of biofilm by Staphylococcus aureus, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae was evaluated in vitro by means of spectrophotometry after growth in microplates and staining with crystalviolet. Of the isolates from intraoperative samples, 44.7% were either moderate or strong biofilm producers compared with 27% of isolates at 6 months after surgery. A decrease in biofilm production was observed for H. influenzae and S. aureus. In conclusion, the rate of isolation and ability to form biofilm decreased in bacteria isolated subsequent to adenoidectomy and/or tonsillectomy. This suggests a role for biofilm in pathogenesis of recurrent and chronic pharyngeal diseases and rhinopharingitis.

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