Investigation of Acinetobacter baumannii resistance to carbapenems in Marseille hospitals, south of France: a transition from an epidemic to an endemic situation
Version of Record online: 28 JUN 2012
© 2012 The Authors APMIS © 2012 APMIS
Volume 121, Issue 1, pages 64–71, January 2013
How to Cite
Kempf M, Rolain J-M, Azza S, Diene S, Joly-Guillou M-L, Dubourg G, Colson P, Papazian L, Richet H, Fournier P-E, Ribeiro A, Raoult D. Investigation of Acinetobacter baumannii resistance to carbapenems in Marseille hospitals, south of France: a transition from an epidemic to an endemic situation. APMIS 2013; 121: 64–71.
- Issue online: 21 DEC 2012
- Version of Record online: 28 JUN 2012
- Manuscript Accepted: 18 MAY 2012
- Manuscript Received: 29 FEB 2012
- Centre National de la Recherche Scientifique
- Acinetobacter baumannii ;
- multidrug resistant bacteria;
- pulsed-field gel electrophoresis;
- bla OXA23-like ;
Carbapenem-resistant Acinetobacter baumannii infections are a worldwide endemic nosocomial threat. Between December 2010 and April 2011, an increase of carbapenem-resistant A. baumannii infections occurred in several Marseille University Hospitals. The aim of this study was to investigate the increase of carbapenem-resistant A. baumannii infections and to characterize the mechanisms of carbapenem resistance. The increase was detected by a homemade computer surveillance program, known as EPIMIC, that monitors antibiotic resistance profiles on a weekly basis. During this period, positive samples of carbapenem-resistant A. baumannii were retrieved from patients hospitalized in different units. Genotyping of the isolates was performed using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), and carbapenemase gene analyses were performed to detect the presence of carbapenemases and to determine the relationships of the isolates. Carbapenem-resistant A. baumannii were isolated in a total of 11 patients who were hospitalized in different hospitals units. We identified the presence of the blaOXA23-like carbapenemase-encoding gene in all of the isolates and found four major PFGE groups and different MLST groups. These results demonstrate a current evolution in the A. baumannii epidemiology in Marseille with a switch from an epidemic situation to an endemic situation and with several circulating clones.