Histological and immunohistochemical analysis of initial and early osseous integration at chemically modified and conventional SLA® titanium implants: preliminary results of a pilot study in dogs
Article first published online: 30 APR 2007
Clinical Oral Implants Research
Volume 18, Issue 4, pages 481–488, August 2007
How to Cite
Schwarz, F., Herten, M., Sager, M., Wieland, M., Dard, M. and Becker, J. (2007), Histological and immunohistochemical analysis of initial and early osseous integration at chemically modified and conventional SLA® titanium implants: preliminary results of a pilot study in dogs. Clinical Oral Implants Research, 18: 481–488. doi: 10.1111/j.1600-0501.2007.01341.x
- Issue published online: 30 APR 2007
- Article first published online: 30 APR 2007
- Date: Accepted 7 May 2006
- animal study;
- histomorphometric analysis;
- immunohistochemical analysis;
- sandblasted and acid-etched surface;
- titanium surface
Objectives: The aim of the present pilot study was to investigate initial and early tissue reactions to modified (mod) and conventional sand-blasted, large grit and acid-etched (SLA) titanium implants.
Material and methods: Implantation of modSLA and SLA implants was performed bilaterally in both the mandible and maxilla of dogs. The animals were sacrificed after a healing period of 1, 4, 7 and 14 days, respectively. Peri-implant tissue reactions were assessed in non-decalcified tissue sections using conventional histology (Toluidine blue-TB and Masson Goldner Trichrome stain-MG) and immunohistochemistry using monoclonal antibodies to transglutaminase II (TG) (angiogenesis) and osteocalcin (OC). Bone density (BD) and bone to implant contact (BIC) were assessed histomorphometrically.
Results: Day 1 revealed an early TG antigen reactivity in the provisional fibrin matrix adjacent to both implant surfaces. Day 4 was characterized by the formation of a collagen-rich connective tissue (MG), which revealed the first signs of OC synthesis adjacent to modSLA surfaces. Immunohistochemical staining for TG revealed a direct correlation between angiogenesis and new bone formation, which was clearly identifiable after 7 days by means of increasing BD, BIC and OC values. After 14 days, modSLA surfaces seemed to be surrounded by a firmly attached mature, parallel-fibered woven bone.
Conclusions: Within the limits of the present study, it might be concluded that the combination of immunohistochemical and conventional histological stainings in non-decalcified tissue sections is a valuable technique to evaluate the initial and early stages of wound healing around endosseous titanium implants.