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Bone morphogenetic protein-2 enhances bone formation when delivered by a synthetic matrix containing hydroxyapatite/tricalciumphosphate

  1. Ronald E. Jung1,
  2. Franz E. Weber2,
  3. Daniel S. Thoma1,
  4. Martin Ehrbar2,
  5. David L. Cochran3,
  6. Christoph H. F. Hämmerle1

Article first published online: 6 DEC 2007

DOI: 10.1111/j.1600-0501.2007.01431.x

Issue

Clinical Oral Implants Research

Clinical Oral Implants Research

Volume 19, Issue 2, pages 188–195, February 2008

Additional Information

How to Cite

Jung, R. E., Weber, F. E., Thoma, D. S., Ehrbar, M., Cochran, D. L. and Hämmerle, C. H. F. (2008), Bone morphogenetic protein-2 enhances bone formation when delivered by a synthetic matrix containing hydroxyapatite/tricalciumphosphate. Clinical Oral Implants Research, 19: 188–195. doi: 10.1111/j.1600-0501.2007.01431.x

Author Information

  1. 1

    Department of Fixed and Removable Prothodontics and Dental Material Science, Dental School, University of Zurich, Zurich, Switzerland

  2. 2

    Oral Biology, Section Bioengineering and Department of Craniomaxillofacial Surgery, University Hospital Zurich, Zurich, Switzerland

  3. 3

    Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

*Correspondence to: Dr Ronald E. Jung Department of Fixed and Removable Prosthodontics and Dental Material Science Dental School University of Zurich Plattenstrasse, 11 CH-8032 Zurich Switzerland Tel.: +41 44 634 32 51 Fax: +41 44 634 43 05 e-mail: jung@zzmk.unizh.ch

Publication History

  1. Issue published online: 6 DEC 2007
  2. Article first published online: 6 DEC 2007
  3. Date: Accepted 29 December 2006

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Keywords:

  • bone morphogenetic protein;
  • bone regeneration;
  • calcium phosphates;
  • carrier material;
  • hydroxyapatite;
  • polyethylene glycol

Abstract

Purpose: The aim of the present study was to test whether or not a synthetic matrix consisting of a polyethylene glycol (PEG) hydrogel containing recombinant human bone morphogenetic protein-2 (rhBMP-2) combined with grafting materials enhances bone regeneration compared with grafting alone or empty control sites.

Material and methods: In each of 10 rabbits, four titanium cylinders were screwed in perforated slits made in the external cortical bones of the calvaria. The following four treatment modalities were randomly allocated: (1) empty control, (2) a combination of a PEG matrix and hydroxyapatite/tricalciumphosphate (HA/TCP) granules and a combination of a PEG matrix containing either 10 μg/ml (3) or 30 μg/ml (4) of BMP-2 and HA/TCP granules. After 8 weeks, the animals were sacrificed and ground sections were obtained for histological analysis. For statistical analysis repeated measures ANOVA and subsequent pairwise Student's t-test were applied (P<0.01).

Results: Histomorphometric analysis showed an average area fraction of newly formed bone of 13.96±5.98% for the empty control, 15.16±7.95% for the PEG and HA/TCP group, 26.32±8.56% for the group containing 10 μg rhBMP-2/ml, and 30.15±7.63% for the group containing 30 μg rhBMP-2/ml. Statistical analysis revealed significantly more newly formed bone in the two rhBMP-2 groups compared with the PEG and HA/TCP group and with the empty control. Regarding the surface fraction of the HA/TCP graft particles covered with newly formed bone the addition of rhBMP-2 revealed a more than two-fold increase compared with cylinders containing HA/TCP granules without rhBMP-2. This difference reached statistical significance.

Conclusions: It is concluded that rhBMP-2 significantly enhances bone regeneration in rabbits when delivered by a synthetic matrix containing HA/TCP. This synthetic PEG matrix containing HA/TCP granules apparently fulfills a number of criteria required for an ideal carrier system for rhBMP-2.

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