The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production

  1. Paolo Amerio1,
  2. Giovina Vianale1,
  3. Marcella Reale1,
  4. Raffaella Muraro1,
  5. Antonello Tulli1,
  6. Adriano Piattelli2

Article first published online: 19 MAR 2010

DOI: 10.1111/j.1600-0501.2009.01881.x

Issue

Clinical Oral Implants Research

Clinical Oral Implants Research

Volume 21, Issue 6, pages 650–655, June 2010

Additional Information

How to Cite

Amerio, P., Vianale, G., Reale, M., Muraro, R., Tulli, A. and Piattelli, A. (2010), The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production. Clinical Oral Implants Research, 21: 650–655. doi: 10.1111/j.1600-0501.2009.01881.x

Author Information

  1. 1

    Department of Oncology and Neuroscience, University of Chieti-Pescara, Chieti, Italy

  2. 2

    Dental School, University of Chieti-Pescara, Chieti, Italy

*Correspondence to: Paolo Amerio Dipartimento di Oncologia e Neuroscienze Clinica Dermatologica Università G. D'Annunzio Via dei Vestini 66013 Chieti Italy Tel.: +39 08 713 58040 Fax: +39 08 715 50157 e-mail: p.amerio@unich.it

Publication History

  1. Issue published online: 11 MAY 2010
  2. Article first published online: 19 MAR 2010
  3. Date: Accepted 17 October 2009

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Keywords:

  • bone substitute;
  • bovine bone;
  • cytokines;
  • growth factors;
  • inflammation

Abstract

Objective: To test the ability of Bio-Oss® in inducing growth factors and proinflammatory cytokines that may have a role in inflammation after grafting, bone resorption, remodeling and in the homeostasis of osteoblasts.

Material and methods: Normal human osteoblasts were seeded in Petri dishes containing granules of Bio-Oss®, cells were harvested after confluency and RNA was extracted. Reverse transcriptase polymerase chain reaction was performed using specific primers for osteonectin, bone sialoprotein (BSP), bone morphogenetic protein (BMP)-2 and BMP-7, platelet-derived growth factor (PDGF), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and integrin β1. Glycerol-3-phosphate dehydrogenase was used as the housekeeping gene and normal human osteoblasts grown on Petri dishes without Bio-Oss® granules were used as negative controls.

Results: Osteoblast grown on Bio-Oss® showed a normal RNA expression of osteonectin, integrin β1 and PDGF. However, compared with control osteoblasts it showed a reduced expression of BSP, BMP-2 and BMP-7, IL-6 and TNF-α.

Conclusions: Our findings further support the evidence that Bio-Oss® is an excellent biomaterial that does not enhance the production of proinflammatory cytokines.

To cite this article: Amerio P, Vianale G, Reale M, Muraro R, Tulli A, Piattelli A. The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production. Clin. Oral Impl. Res. 21, 2010; 650–655. doi: 10.1111/j.1600-0501.2009.01881.x

View Full Article (HTML) Get PDF (275K)