Enhanced bone regeneration around dental implant with bone morphogenetic protein 2 gene and vascular endothelial growth factor protein delivery

Authors


Corresponding authors:
Xiangrong Cheng
Ministry Education Key Laboratory for Oral Biomedical Engineering
School of Stomatology
Wuhan University
Wuhan 430079
China
Tel.: +86 27 8764 6351
Fax: +86 27 8787 3260
e-mail: xiangrongcheng@hotmail.com
Yufeng Zhang
Ministry Education Key Laboratory for Oral Biomedical Engineering
School of Stomatology
Wuhan University
Wuhan 430079
China
Tel.: +86 27 8768 6104
Fax: +86 27 8787 3260
e-mail: zyf@whu.edu.cn

Abstract

Objective: To evaluate the synergistic effect of bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) on the repair of bone defects around dental implants.

Material and methods: Five groups of scaffold were fabricated by a freeze-drying method, including pure chitosan/collagen scaffold; scaffold loaded with adenoviruses expressing BMP-2, adenoviruses expressing VEGF, both adenoviruses expressing BMP-2 and VEGF, VEGF protein and adenovirus expressing BMP-2. In vitro studies examined whether bone marrow stromal cells were responsive to these scaffolds over time. Bone formation capacity, bone-to-implant contact, as well as removal torque values were investigated in vivo. Differences between the various groups were statistically analyzed using the one-way analysis of variance test.

Results: The in vitro study revealed a burst and rapid release of VEGF with a sustained high-level expression of BMP-2 in scaffold combined with VEGF protein and adenoviruses expressing BMP-2. Histomorphometry demonstrated that scaffolds expressing BMP-2 enhanced more bone formation compared with other groups; VEGF alone is insufficient to promote bone formation. New bone formation in the bone defects around dental implants, bone-to-implant contact and mean peak removal torque showed statistically significant difference for the adenoviral vector encoding human bone morphogenetic protein 2 (Ad-BMP-2) and VEGF protein and adenovirus expressing BMP-2 groups. Furthermore, scaffold combined with VEGF protein and Ad-BMP-2 represented the best outcomes in this model.

Conclusions: A combination of BMP-2 gene and VEGF protein could have a synergistic effect in promoting bone healing.

To cite this article:
Luo T, Zhang W, Shi B, Cheng X, Zhang Y. Enhanced bone regeneration around dental implant with bone morphogenetic protein 2 gene and vascular endothelial growth factor protein delivery.
Clin. Oral Impl. Res. 23, 2012 467–474.
doi: 10.1111/j.1600-0501.2011.02164.x

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