Transforming growth factor-β stimulates Interleukin-11 production by human periodontal ligament and gingival fibroblasts

Authors

  • R. Yashiro,

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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  • T. Nagasawa,

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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  • M. Kiji,

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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  • D. Hormdee,

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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  • H. Kobayashi,

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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  • G. Koshy,

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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  • H. Nitta,

    1. Behavioral Dentistry, Department of Comprehensive Oral Health Care, Tokyo Medical and Dental University, Tokyo, Japan
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  • I. Ishikawa

    1. Periodontology, Department of Hard Tissue Engineering, Graduate School
    2. Centre of Excellence Program for Frontier Research on Molecular Destruction of Tooth and Bone and;
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Address:
R. Yashiro
Periodontology Department of Hard Tissue Engineering
Graduate School, Tokyo Medical and Dental University
1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549 Japan
E-mail: reiko-yashiro.peri@tmd.ac.jp

Abstract

Background: Transforming growth factor (TGF)-β is a potent multifunctional polypeptide, abundant in the bone matrix. Interleukin (IL)-11 is a pleiotropic cytokine with effects on multiple cell types. The present study was performed to evaluate the regulatory effects of TGF-β on IL-11 production by human periodontal ligament cells (PDL) and human gingival fibroblasts (HGF).

Material and Methods: The expression of TGF-β receptor in PDL and HGF were observed using flow cytometry. PDL and HGF were stimulated with TGF-β with or without protein kinase C (PKC) inhibitors and activator. IL-11, bone morphogenetic protein-2 (BMP-2) and TGF-β mRNA expression was quantified by real-time polymerase chain reaction (PCR). IL-11 production was measured using enzyme-linked immunosorbent assay.

Results: PDL and HGF expressed both TGF-β receptor I and TGF-β receptor II on the cell surfaces. IL-11 mRNA expression and IL-11 production were augmented by TGF-β in both PDL and HGF, with higher values in PDL. PKC inhibitors partially suppressed TGF-β-induced IL-11 production in PDL and HGF, whereas activator enhanced it. TGF-β mRNA and BMP-2 mRNA expression were up-regulated by TGF-β in PDL.

Conclusion: These results suggest that PDL produce IL-11 in response to TGF-β.

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