Adiposity and gingival crevicular fluid tumour necrosis factor-α levels in children


  • Conflict of interest and sources of funding statement
    This study was supported by Fonds de la recherche en santé du Québec (FRSQ) and Canadian Institutes of Health Research (CIHR). Roozbeh Khosravi is the recipient of a MSc fellowship from the Armand-Frappier Foundation. Belinda Nicolau holds a New Investigator Award from the CIHR.

Dr. Belinda Nicolau
Epidemiology & Biostatistics Unit
INRS-Institut Armand-Frappier
531 boul. des Prairies
Canada H7V 1B7


Aim: To investigate whether adiposity is associated with gingival crevicular fluid (GCF) tumour necrosis factor-α (TNF-α) levels in children. We also examined whether this relationship is mediated through plasma fasting insulin.

Materials and Methods: This preliminary study used cross-sectional data from the baseline-visit of the Quebec Adipose and Lifestyle InvesTigation in Youth cohort, which is an ongoing longitudinal study investigating the natural history of obesity in Quebec children. Study participants (76 girls and 102 boys) include children aged 8–10 years and their families, living in the Montreal and Quebec City areas. TNF-α level was measured in pooled samples (N=4) for each child by enzyme-linked immunosorbant assay. Height and weight were measured. Body mass index (BMI) was calculated as weight/height2 (kg/m2). Sex/age-specific BMI was categorized into normal (<85th percentile), overweight (85th–95th percentile) and obese (geqslant R: gt-or-equal, slanted95th percentile) defined by the 2000 US-CDC growth charts. Insulin resistance was measured using fasting plasma insulin in children. Data analysis involved descriptive and multiple linear regression analyses.

Results: Our results suggest that obesity in boys was associated with a 37% increase of GCF-TNF-α level. However, when accounting for insulin resistance this association was reduced and disappeared while the model's goodness of fit improved.

Conclusions: These findings provide support for the link between adiposity in children and GCF-TNF-α level, which appears to be mediated by insulin resistance.