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Salivary MMP-8, TIMP-1, and ICTP as markers of advanced periodontitis

Authors

  • Ulvi Kahraman Gursoy,

    1. Institute of Dentistry, University of Helsinki, Helsinki, Finland
    2. Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, Helsinki, Finland
    3. Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Helsinki, Finland
    4. Institute of Dentistry, University of Turku, Turku, Finland
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  • Eija Könönen,

    1. Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Helsinki, Finland
    2. Institute of Dentistry, University of Turku, Turku, Finland
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  • Pratikshya Pradhan-Palikhe,

    1. Institute of Dentistry, University of Helsinki, Helsinki, Finland
    2. Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, Helsinki, Finland
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  • Taina Tervahartiala,

    1. Institute of Dentistry, University of Helsinki, Helsinki, Finland
    2. Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, Helsinki, Finland
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  • Pirkko J. Pussinen,

    1. Institute of Dentistry, University of Helsinki, Helsinki, Finland
    2. Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, Helsinki, Finland
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  • Liisa Suominen-Taipale,

    1. Institute of Dentistry, University of Turku, Turku, Finland
    2. Unit of Living Conditions, Health and Wellbeing, Department of Environmental Health in Environmental Epidemiology Unit, National Institute for Health and Welfare (THL), Kuopio, Finland
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  • Timo Sorsa

    1. Institute of Dentistry, University of Helsinki, Helsinki, Finland
    2. Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, Helsinki, Finland
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  • Conflict of interest and sources of funding statement
    The authors declare that they have no conflicts of interests.
    The present study is a part of the Health 2000 Health Examination survey, organized by National Institute for Health and Welfare of Finland. This study was supported by grants from the Paulo and Yrjo Jahnsson Foundations (for U. K. G.), the Academy of Finland (grant 78443 for E. K. and grant 118391 for P. J. P.), and the Helsinki University Central Hospital Research Foundation. Timo Sorsa is the inventor of US patents 5652227, 5736341, 5866432, and 6143476.

Address:
Ulvi Kahraman Gursoy
Institute of Dentistry
University of Helsinki
Biomedicum
PO Box 63
00014 Helsinki
Finland
E-mail: ulvi.gursoy@helsinki.fi; ulvi.gursoy@utu.fi

Abstract

Gursoy UK, Könönen E, Pradhan-Palikhe P, Tervahartiala T, Pussinen PJ, Suominen-Taipale L, Sorsa T. Salivary MMP-8, TIMP-1, and ICTP as markers of advanced periodontitis. J Clin Periodontol 2010; 37: 487–493. doi: 10.1111/j.1600-051X.2010.01563.x.

Abstract

Aim: Salivary matrix metalloproteinase (MMP)-8 and -14, tissue inhibitor of matrix metalloproteinase (TIMP)-1, and pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP) were analysed aiming to detect potential markers of advanced periodontitis in saliva. In addition, we compared two MMP-8 detection methods, a time-resolved immunofluorometric assay (IFMA) and an enzyme-linked immunoassay (ELISA), to differentiate periodontitis subjects from controls.

Material and Methods: Concentrations of MMP-8, MMP-14, TIMP-1, and ICTP were analysed from salivary specimens of 165 subjects, including 84 subjects having at least 14 teeth with periodontal pocket (pocket depth geqslant R: gt-or-equal, slanted4 mm) and 81 subjects without pocket depth as their controls.

Results: Salivary MMP-8 detection by IFMA differentiated periodontitis subjects from controls more strongly than by ELISA. Salivary MMP-8, TIMP-1, and ICTP concentrations were higher in periodontitis subjects than those in controls. When only smokers were included in the analysis these differences were lost. The MMP-8/TIMP-1 ratio and the combination of MMP-8 and ICTP differentiated periodontitis and control groups even in smoker subjects.

Conclusion: Salivary MMP-8, TIMP-1, ICTP, and especially their ratios and combinations are potential candidates in the detection of advanced periodontitis. Differentiating periodontitis and control subjects with salivary MMP-8 detection is dependent on the selected techniques.

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