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Journal of Clinical Periodontology

Salivary proteome modifications associated with periodontitis in obese patients

Authors

  • Hélène Rangé,

    1. Department of Periodontology, Service of Odontology, Garancière Rothschild Hospital, AP-HP, Paris 7-Denis Diderot University, U.F.R. of Odontology, Paris, France
    2. INSERM Unit 698, Cardiovascular, Hematology, Bio Engineering and Remodeling, Bichat Hospital, AP-HP, Paris 7-Denis Diderot University, Paris, France
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  • Thibaut Léger,

    1. INSERM Unit 698, Cardiovascular, Hematology, Bio Engineering and Remodeling, Bichat Hospital, AP-HP, Paris 7-Denis Diderot University, Paris, France
    2. Plateforme Protéomique Structurale et Fonctionnelle, Institut Jacques Monod – UMR 7592 Paris 7-Denis Diderot University -CNRS, Paris, France
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  • Clarisse Huchon,

    1. Service of Odontology, Bretonneau Hospital, AP-HP, Paris 5-Descartes University, EA 2496 U.F.R. of Odontology, Paris, France
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  • Cécile Ciangura,

    1. Department of Nutrition, Pitié-Salpêtrière Hospital AP-HP, Paris, France
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  • Devy Diallo,

    1. INSERM Unit 698, Cardiovascular, Hematology, Bio Engineering and Remodeling, Bichat Hospital, AP-HP, Paris 7-Denis Diderot University, Paris, France
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  • Christine Poitou,

    1. Department of Nutrition, Pitié-Salpêtrière Hospital AP-HP, Paris, France
    2. Paris 6-Pierre et Marie Curie University, Human Nutrition Research Center Ile-de-France (CRNH IdF), Paris, France
    3. INSERM Unit 872, Team 7 Nutriomique, Cordeliers Research Center, Paris, France
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  • Olivier Meilhac,

    1. INSERM Unit 698, Cardiovascular, Hematology, Bio Engineering and Remodeling, Bichat Hospital, AP-HP, Paris 7-Denis Diderot University, Paris, France
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  • Philippe Bouchard,

    Corresponding author
    • Department of Periodontology, Service of Odontology, Garancière Rothschild Hospital, AP-HP, Paris 7-Denis Diderot University, U.F.R. of Odontology, Paris, France
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  • Catherine Chaussain

    1. Service of Odontology, Bretonneau Hospital, AP-HP, Paris 5-Descartes University, EA 2496 U.F.R. of Odontology, Paris, France
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  • Conflict of interest and source of funding statement

    The authors declare that they have no conflict of interest. This research project was supported by grants from Assistance Publique-Hôpitaux de Paris (AP-HP), GABA France and Colgate – Palmolive Europe.

Address:

Philippe Bouchard

U.F.R. d'Odontologie

5, rue Garancière

75006 Paris

France

E-mail: phbouch@noos.fr

Abstract

Aim

To identify changes in the salivary protein/peptide profiles by differential proteomics in obese patients with or without periodontitis.

Material and Methods

Periodontal examinations and whole saliva samples were obtained from 38 obese patients (mean age: 45.1 ± 7.3 years, mean BMI: 49.3 ± 9 kg/m2) including 13 periodontitis and 25 non-periodontitis subjects, and 19 healthy controls (mean age: 44.2 ± 6.4 years, mean BMI: 21.5 ± 2.1 kg/m2). Surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS) was used to compare the whole saliva polypeptide profiles.

Results

The SELDI-TOF-MS analysis detected eight putative markers. Six of them were increased and identified in obese subjects versus controls (albumin, α and β haemoglobin chains, α-defensins 1, 2 and 3). Alpha-defensins were less abundant in saliva of periodontitis obese patients (36.47 ± 19.84 μA) versus non-periodontitis obese patients (43.44 ± 30.34  μA), whereas α-defensins were more abundant in obese patients (40.99 ± 26.66  μA) versus controls (27.1 ± 23.98  μA).

Conclusions

Periodontal status modifies the salivary proteome in obese patients. Alpha-defensins may play a role in gingival inflammation, and be involved in the higher susceptibility of obese patients to periodontal diseases.

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