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Combined photoablative and photodynamic diode laser therapy as an adjunct to non-surgical periodontal treatment. A randomized split-mouth clinical trial


  • Conflict of interest and source of funding

  • The authors declare that they have no conflict of interest.

  • The study was self-funded by the authors and/or their institution.


Daniele Bani

Section of Histology, Department of Anatomy, Histology & Forensic Medicine

University of Florence

viale G.Pieraccini 6, I-50139, Florence





Comparing the efficacy of photoablative and photodynamic diode laser in adjunct to scaling -root planing (SRP) and SRP alone for the treatment of chronic periodontitis.

Materials and Methods

Twenty-six patients were studied. Maxillary left or right quadrants were randomly assigned to sham-laser treatment + SRP or laser + SRP. This consisted of photoablative intra/extra-pocket de-epithelization with diode laser (λ = 810 nm), followed by single SRP and multiple photodynamic treatments (once weekly, 4–10 applications, mean ± SD: 3.7 ± 2.4) using diode laser (λ = 635 nm) and 0.3% methylene blue as photosensitizer. The patients were monitored at days 0 and 365 by clinical assessment (probing depth, PD; clinical attachment level, CAL; bleeding on probing, BOP) and at days 0, 15, 30, 45, 60, 75, 90, 365 by cytofluorescence analysis of gingival exfoliative samples taken in proximity of the teeth to be treated (polymorphonuclear leukocytes, PMN; red blood cells, RBC; damaged epithelial cells, DEC; bacteria).


At day 365, compared with the control quadrants, the laser + SRP therapy yielded a significant (< 0.001) reduction in PD (−1.9 mm), CAL (−1.7 mm) and BOP (−33.2% bleeding sites), as well as in bacterial contamination – especially spirochetes – and PMN and RBC shedding in the gingival samples (< 0.001).


Diode laser treatment (photoablation followed by multiple photodynamic cycles) adjunctive to conventional SRP improves healing in chronic periodontitis patients.

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