Presented at the Third Conference on Cutaneous Toxicity, Washington, D.C., 18 May 1976.
The chamber-scarification test for irritancy *
Article first published online: 11 APR 2006
© 1976 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 2, Issue 6, pages 314–324, December 1976
How to Cite
Frosch, P. J. and Kligman, A. M. (1976), The chamber-scarification test for irritancy . Contact Dermatitis, 2: 314–324. doi: 10.1111/j.1600-0536.1976.tb03068.x
- Issue published online: 11 APR 2006
- Article first published online: 11 APR 2006
- Received for publication July 19, J976
- chamber method;
- irritancy testing;
- propylene glycol;
A procedure has been designed (the chamber-scarification test), which possesses greatly increased sensitivity for assessing the irritancy of topically applied materials. A forearm test site is criss-cross scarified by drawing a 30-gauge needle over the skin with just enough pressure to cleave the epidermis without drawing blood. The test agent is applied in an aluminum chamber once daily for 3 days.
The advantages of the method over conventional patch testing are: enhanced capacity to measure mild irritants, reduced time (3 days versus 10 to 21 days), less effort, less cost, less discombort for the volunteers and greater reproducibility.