Skin harrier function was studied after use of occlusive gloves on normal and compromised skin. 2 studies were performed (Study A and B), and the effects were evaluated by non-invasive methods. Participants in the studies were instructed to wear an occlusive glove on one hand, while the other hand served as control. The gloves used were hypoallergenic, non-latex. Study A: 20 volunteers wore a glove on normal skin 6 h/day for 3 days. Study B: 20 volunteers wore a glove on sodium fauryl sulfa lei S LSI-compromised skin 6 h/day for 3 days. Skin harrier function was evaluated by measurement of transepidermal water loss (TEWL) (Evaporimeter), skin hydration by electrical capacitance (Corneometer) and inflammation was evaluated by erythema index (DermaSpetrometer). Results: Study A. Glove occlusion on normal skin 6 h/day for 3 days caused no significant influence on the water barrier function. Study B: Glove occlusion on SLS-compromised skin for the same period of time had a significantly negative effect on the water barrier function. It is concluded that occlusion may be an additional factor in the pathogenesis of cumulative irritant contact dermatitis.