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The expression of molecular mediators in the idiopathic cutaneous calcification and ossification

Authors

  • So Young Kim,

    1. Department of Dermatology, School of Medicine, Ewha Womans University, Seoul, Korea
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  • Hae Young Choi,

    Corresponding author
    1. Department of Dermatology, School of Medicine, Ewha Womans University, Seoul, Korea
      Prof Hae Young Choi, Department of Dermatology, School of Medicine, Ewha Womans University, Mokdong Hospital 911-1 Mokdong YangchonGu, 158710 Seoul, Korea
      Tel: +82 2 2650 5159
      Fax: +82 2 2652 6925
      e-mail: hychoi@ewha.ac.kr
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  • Ki Bum Myung,

    1. Department of Dermatology, School of Medicine, Ewha Womans University, Seoul, Korea
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  • You Won Choi

    1. Department of Dermatology, School of Medicine, Ewha Womans University, Seoul, Korea
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Prof Hae Young Choi, Department of Dermatology, School of Medicine, Ewha Womans University, Mokdong Hospital 911-1 Mokdong YangchonGu, 158710 Seoul, Korea
Tel: +82 2 2650 5159
Fax: +82 2 2652 6925
e-mail: hychoi@ewha.ac.kr

Abstract

Background:  Idiopathic cutaneous calcification and ossification occur in the absence of an abnormal serum calcium level or pre-existing tissue abnormality. The pathogenesis has not been fully elucidated. The aim of this study was to investigate the expression of several molecular mediators in the idiopathic cutaneous calcification and ossification.

Methods:  Immunohistochemical study was used to evaluate the expression of molecular mediators, bone morphogenetic protein 4 (BMP-4), β-catenin, osteopontin, osteonectin and osteocalcin, and cell markers, smooth muscle actin, CD29 and CD44. And confocal laser scanning microscopy was used to evaluate the colocalization of BMP-4 and BMP receptor type IA.

Results:  BMP-4, β-catenin, osteopontin, osteonectin and osteocalcin were expressed on the calcified and ossified tissue. Especially, BMP-4 was expressed on the surrounding mesenchymal cells. Smooth muscle actin positive mesenchymal cells were on around the immature ossified tissue. Mesenchymal stem cell markers, CD29 and CD44 were not expressed.

Conclusion:  Our data suggest that BMP-4, β-catenin, osteopontin, osteonectin and osteocalcin may be involved in the idiopathic cutaneous calcification and ossification. And smooth muscle actin positive mesenchymal cells may be involved in the cutaneous ossification. This study suggests that the idiopathic cutaneous calcification and ossification is highly complicated and regulated active process like ectopic calcification of other tissues.

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