Prevalence of MCPyV in Merkel cell carcinoma and non-MCC tumors
Article first published online: 14 JUL 2009
Copyright © 2009 John Wiley & Sons A/S
Journal of Cutaneous Pathology
Volume 37, Issue 1, pages 28–34, January 2010
How to Cite
Andres, C., Belloni, B., Puchta, U., Sander, C. A. and Flaig, M. J. (2010), Prevalence of MCPyV in Merkel cell carcinoma and non-MCC tumors. Journal of Cutaneous Pathology, 37: 28–34. doi: 10.1111/j.1600-0560.2009.01352.x
- Issue published online: 24 NOV 2009
- Article first published online: 14 JUL 2009
- Accepted for publication March 9, 2009
Background: Merkel cell polyomavirus (MCPyV) is the likely causative agent of Merkel cell carcinoma (MCC). However, the prevalence of MCPyV in non-MCC population and its possible role in the pathogenesis of other skin cancers are not known yet.
Methods: A molecular pathology study was performed in 33 MCC samples and 33 age- and sex-matched samples of sun exposed non-MCC tumors [12 seborrheic keratoses (SK), 11 basal cell carcinomas (BCC) and 10 lentigo maligna melanomas (LMM)]. All tumors were analyzed for presence of MCPyV-DNA by polymerase chain reaction (PCR) and Southern-Blot hybridization of PCR products.
Results: MCPyV sequences were detected in 21 MCC samples (64%) and in 2 non-MCC tumors of sun exposed skin (6%; both SK-patients). Neither the tissue samples from BCC nor LMM proved positive for MCPyV sequences.
Conclusion: We were able to confirm prior data on prevalence of MCPyV-DNA in MCC. Furthermore, a female predominance of MCPyV-positive MCC-patients was detected. There was no relevant association of MCPyV with SK, BCC and LMM. Speculative, prevalence of MCPyV in an age- and sex-matched non-MCC population could average up to 6%.