X.-G. Liu, J.-L. Li and P. Qin contributed equally to this manuscript.
Determination of platelet-bound glycoprotein-specific autoantibodies by flow cytometric immunobead assay in primary immune thrombocytopenia
Article first published online: 18 FEB 2011
© 2011 John Wiley & Sons A/S
European Journal of Haematology
Volume 86, Issue 4, pages 339–346, April 2011
How to Cite
Liu, X.-G., Li, J.-L., Qin, P., Ren, J., Ma, S.-H., Sun, L., Shi, Y., Ji, X.-B., Zhu, Y.-Y., Ma, D.-X., Guo, C.-S., Du, X., Hou, M. and Peng, J. (2011), Determination of platelet-bound glycoprotein-specific autoantibodies by flow cytometric immunobead assay in primary immune thrombocytopenia. European Journal of Haematology, 86: 339–346. doi: 10.1111/j.1600-0609.2010.01572.x
- Issue published online: 18 MAR 2011
- Article first published online: 18 FEB 2011
- Accepted manuscript online: 2 JAN 2011 09:18PM EST
- Accepted for publication 29 December 2010
- primary immune thrombocytopenia;
- flow cytometry;
Objectives: Primary immune thrombocytopenia (ITP) is an autoimmune disorder characterized by premature platelet destruction induced by autoantibodies directed against platelet glycoproteins (GPs). Despite being a clinically important disorder, ITP lacks a feasible diagnostic assay for routine clinical use. This study was meant to evaluate a newly developed flow cytometric immunobead assay for determination of platelet-bound GP-specific autoantibodies in comparison with indirect monoclonal antibody-specific immobilization of platelet antigen (MAIPA) in the diagnosis of ITP.
Methods: Platelet-bound and plasma GPIIb/IIIa and GPIb/IX autoantibodies were determined by flow cytometric immunobead assay and indirect modified MAIPA, respectively. The average fluorescence level for platelet-bound, GP-specific autoantibodies was given as a ratio to three normal controls tested simultaneously.
Results: The median value of platelet-bound GPIIb/IIIa and GPIb/IX autoantibodies in ITP group were 3.09 (range 0.78, 30.2) and 3.09 (range 0.72, 19.2), respectively, which were significantly higher than non-ITP group [1.01 (0.67, 5.59) and 1.01 (0.79, 5.56), respectively, P < 0.001] and normal controls [1.02 (0.72, 1.76) and 1.03 (0.79, 1.73), respectively, P < 0.001]. The receiver-operating characteristics curve analysis showed an area under the curve of 0.895 for GPIIb/IIIa autoantibody and 0.859 for GPIb/IX autoantibody, respectively. Combined detection of GPIIb/IIIa or GPIb/IX autoantibodies by flow cytometric immunobead assay showed a sensitivity of 82.11% for ITP diagnosis.
Conclusion: This study demonstrated that determination of platelet-bound, GP-specific autoantibodies by flow cytometric immunobead assay was a convenient, sensitive, and specific test for the differential diagnosis of thrombocytopenic patients.