Get access

Expression of thermosensitive two-pore domain K+ channels in human keratinocytes cell line HaCaT cells

Authors

  • Dawon Kang,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Sung-Hee Kim,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Eun-Mi Hwang,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Oh-Sang Kwon,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Hae-Young Yang,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Eun-Sook Kim,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Tae Hyun Choi,

    1. Department of Plastic and Reconstructive Surgery, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Jae-Yong Park,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Seong-Geun Hong,

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author
  • Jaehee Han

    1. Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea
    Search for more papers by this author

Jaehee Han, MD, Ph.D., Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, 90 Chilam-dong, Jinju, Gyeongsangnam-do 660-751 Korea, Tel.: 82 55 751 8742, Fax: 82 55 759 0169, e-mail: jheehan@gnu.ac.kr

Abstract

Abstract:  Recent studies have shown that keratinocytes can sense temperature via thermo-transient receptor potential (TRP) channels. It is not known whether other thermosensitive ion channels such as TREK-1, TREK-2 and TRAAK (TREKs/TRAAK) that are members of the two-pore domain K+ (K2P) channel family are expressed in human keratinocytes. Here, we identified the expression of TREKs/TRAAK in human keratinocytes-derived cell line HaCaT cells using RT-PCR, immunocytochemistry, Western blot analysis and patch-clamp technique. RT-PCR showed that all six K2P channels tested (TASK-1, TASK-3, TREK-1, TREK-2, TRAAK and TASK-2) were expressed in HaCaT cells, as well as in skin and dorsal root ganglion (DRG) of rat. The expression of TREKs/TRAAK mRNA identified by RT-PCR was further studied at the protein level. Using anti-TREK-1, -TREK-2 and -TRAAK, bands of ∼46, ∼60 and ∼43 kDa, respectively, were observed at plasma membrane of HaCaT cells. Immunostaining also showed that TREK-1, TREK-2 and TRAAK were expressed in all area of cells including plasma membrane. Whole-cell K+ currents recorded from HaCaT cells were activated by arachidonic acid and heat. These results suggest that TREKs/TRAAK channels could act as thermosensors in human keratinocytes.

Ancillary